Matches in SemOpenAlex for { <https://semopenalex.org/work/W72576449> ?p ?o ?g. }
- W72576449 endingPage "19542" @default.
- W72576449 startingPage "19535" @default.
- W72576449 abstract "The periplasmic histidine transport system (permease) of Escherichia coli and Salmonella typhimurium is composed of a soluble, histidine-binding receptor located in the periplasm and a complex of three membrane-bound proteins of which one, HisP, was shown previously to bind ATP. These permeases are energized by ATP. HisP is a member of a family of membrane transport proteins which is conserved in all periplasmic permeases and is presumed to be involved in coupling the energy of ATP to periplasmic transport. In this paper the nature of the ATP-binding site of HisP has been explored by identification of some of the residues that come into contact with ATP. HisP was derivatized with 8-azido-ATP (N3ATP). Both the underivatized and the derivatized forms of HisP were solubilized, purified, and digested with trypsin. The resulting tryptic peptides were resolved by high pressure liquid chromatography, and peptides modified by N3ATP were isolated and sequenced. Two peptides, X and Z, spanning amino acid residues 16-23 and 31-45, were found to contain sites of N3ATP attachment at His19 and Ser41, respectively. Both peptides are close to the amino-terminal end of HisP; peptide Z is located in one of the well conserved regions comprising the nucleotide-binding consensus motifs of the energy-coupling components of these permeases. These consensus motifs are found in many purine nucleotide-binding proteins. The relationship between the location of these residues and the overall structure of the ATP-binding site is discussed." @default.
- W72576449 created "2016-06-24" @default.
- W72576449 creator A5013095461 @default.
- W72576449 creator A5046685224 @default.
- W72576449 creator A5076790983 @default.
- W72576449 creator A5089087944 @default.
- W72576449 date "1990-11-01" @default.
- W72576449 modified "2023-09-28" @default.
- W72576449 title "The nucleotide-binding site of HisP, a membrane protein of the histidine permease. Identification of amino acid residues photoaffinity labeled by 8-azido-ATP." @default.
- W72576449 cites W1139343036 @default.
- W72576449 cites W124504601 @default.
- W72576449 cites W1416966580 @default.
- W72576449 cites W1480789058 @default.
- W72576449 cites W1488014323 @default.
- W72576449 cites W1528987874 @default.
- W72576449 cites W1554937054 @default.
- W72576449 cites W1559005407 @default.
- W72576449 cites W1569916926 @default.
- W72576449 cites W1581124244 @default.
- W72576449 cites W1597599526 @default.
- W72576449 cites W1597691924 @default.
- W72576449 cites W1599579794 @default.
- W72576449 cites W1606821594 @default.
- W72576449 cites W1665931340 @default.
- W72576449 cites W1673579390 @default.
- W72576449 cites W1847457361 @default.
- W72576449 cites W1880075813 @default.
- W72576449 cites W1929877158 @default.
- W72576449 cites W1989218766 @default.
- W72576449 cites W1989675515 @default.
- W72576449 cites W1996272077 @default.
- W72576449 cites W1997497266 @default.
- W72576449 cites W2013973825 @default.
- W72576449 cites W2024103839 @default.
- W72576449 cites W2031332665 @default.
- W72576449 cites W2031683521 @default.
- W72576449 cites W2039358467 @default.
- W72576449 cites W2050039518 @default.
- W72576449 cites W2058852380 @default.
- W72576449 cites W2060455791 @default.
- W72576449 cites W2065123532 @default.
- W72576449 cites W2071220601 @default.
- W72576449 cites W2083812900 @default.
- W72576449 cites W2097878215 @default.
- W72576449 cites W2100837269 @default.
- W72576449 cites W2102131116 @default.
- W72576449 cites W2130116578 @default.
- W72576449 cites W2145899653 @default.
- W72576449 cites W2146100256 @default.
- W72576449 cites W2167859394 @default.
- W72576449 cites W2180739100 @default.
- W72576449 cites W2313965973 @default.
- W72576449 cites W2743792571 @default.
- W72576449 cites W35929032 @default.
- W72576449 cites W47210259 @default.
- W72576449 doi "https://doi.org/10.1016/s0021-9258(17)45405-6" @default.
- W72576449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2246240" @default.
- W72576449 hasPublicationYear "1990" @default.
- W72576449 type Work @default.
- W72576449 sameAs 72576449 @default.
- W72576449 citedByCount "29" @default.
- W72576449 crossrefType "journal-article" @default.
- W72576449 hasAuthorship W72576449A5013095461 @default.
- W72576449 hasAuthorship W72576449A5046685224 @default.
- W72576449 hasAuthorship W72576449A5076790983 @default.
- W72576449 hasAuthorship W72576449A5089087944 @default.
- W72576449 hasBestOaLocation W725764491 @default.
- W72576449 hasConcept C104317684 @default.
- W72576449 hasConcept C107824862 @default.
- W72576449 hasConcept C149011108 @default.
- W72576449 hasConcept C185592680 @default.
- W72576449 hasConcept C190114821 @default.
- W72576449 hasConcept C2778460671 @default.
- W72576449 hasConcept C2778581200 @default.
- W72576449 hasConcept C41625074 @default.
- W72576449 hasConcept C512185932 @default.
- W72576449 hasConcept C515207424 @default.
- W72576449 hasConcept C55493867 @default.
- W72576449 hasConcept C71240020 @default.
- W72576449 hasConceptScore W72576449C104317684 @default.
- W72576449 hasConceptScore W72576449C107824862 @default.
- W72576449 hasConceptScore W72576449C149011108 @default.
- W72576449 hasConceptScore W72576449C185592680 @default.
- W72576449 hasConceptScore W72576449C190114821 @default.
- W72576449 hasConceptScore W72576449C2778460671 @default.
- W72576449 hasConceptScore W72576449C2778581200 @default.
- W72576449 hasConceptScore W72576449C41625074 @default.
- W72576449 hasConceptScore W72576449C512185932 @default.
- W72576449 hasConceptScore W72576449C515207424 @default.
- W72576449 hasConceptScore W72576449C55493867 @default.
- W72576449 hasConceptScore W72576449C71240020 @default.
- W72576449 hasIssue "32" @default.
- W72576449 hasLocation W725764491 @default.
- W72576449 hasOpenAccess W72576449 @default.
- W72576449 hasPrimaryLocation W725764491 @default.
- W72576449 hasRelatedWork W1498644340 @default.
- W72576449 hasRelatedWork W1625235052 @default.
- W72576449 hasRelatedWork W1697966926 @default.