Matches in SemOpenAlex for { <https://semopenalex.org/work/W72647192> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W72647192 endingPage "2480" @default.
- W72647192 startingPage "2466" @default.
- W72647192 abstract "Lymphatic filariasis is a major tropical disease caused by mosquito born nematodes Brugia malayi and Wuchereria bancrofti. Vaccine against filariasis must generate immunity to infective mosquito derived L3 stage. Two highly expressed genes designated abundant larval transcript-1 and -2 (alt-1 and alt-2). ALT-1 and ALT-2 represent closely related protein (79%) it. Now, expression of this alt gene in E. coli BL21plysS for the production of vaccine is major challenge as no vaccine is available against this disease. Work was carried out to express this protein at laboratory scale bioreactor. At first optimization of different parameter like suitability of media, inducer concentration, induction time was done for getting maximum amount of recombinant protein. In shake flask studies, after induction (max cell density and max specific growth rate stage) good expression of ALT-2 protein was found. However, at laboratory scale production done in bioreactor, expression level drastically decreased. Plasmid stability analysis was done in reactor and was found to be cause for decreased productivity. The stability was improved by increasing antibiotic concentration in the medium and also by pulsing antibiotic during induction. This led to better plasmid stability and increased expression levels in reactor similar to expression levels in shake flask studies. JMBFS / Asharf et al. 2013 : 2 (4) 2466-248" @default.
- W72647192 created "2016-06-24" @default.
- W72647192 creator A5027706708 @default.
- W72647192 creator A5044977802 @default.
- W72647192 creator A5050011092 @default.
- W72647192 creator A5071457470 @default.
- W72647192 date "2013-01-01" @default.
- W72647192 modified "2023-09-22" @default.
- W72647192 title "Recombinant protein production of abundant larval transcript (ALT-2) in Escherichia coli." @default.
- W72647192 cites W107610474 @default.
- W72647192 cites W1881586736 @default.
- W72647192 cites W1968157175 @default.
- W72647192 cites W1985077925 @default.
- W72647192 cites W1991486518 @default.
- W72647192 cites W1995686191 @default.
- W72647192 cites W2001751900 @default.
- W72647192 cites W2006546142 @default.
- W72647192 cites W2019905403 @default.
- W72647192 cites W2021731334 @default.
- W72647192 cites W2053448984 @default.
- W72647192 cites W2055059689 @default.
- W72647192 cites W2063790877 @default.
- W72647192 cites W2086652584 @default.
- W72647192 cites W2100837269 @default.
- W72647192 cites W2110039658 @default.
- W72647192 cites W2137490166 @default.
- W72647192 cites W2152732370 @default.
- W72647192 cites W2162801432 @default.
- W72647192 cites W2207132703 @default.
- W72647192 cites W2268560891 @default.
- W72647192 cites W4366438 @default.
- W72647192 hasPublicationYear "2013" @default.
- W72647192 type Work @default.
- W72647192 sameAs 72647192 @default.
- W72647192 citedByCount "0" @default.
- W72647192 crossrefType "journal-article" @default.
- W72647192 hasAuthorship W72647192A5027706708 @default.
- W72647192 hasAuthorship W72647192A5044977802 @default.
- W72647192 hasAuthorship W72647192A5050011092 @default.
- W72647192 hasAuthorship W72647192A5071457470 @default.
- W72647192 hasConcept C104317684 @default.
- W72647192 hasConcept C150194340 @default.
- W72647192 hasConcept C153911025 @default.
- W72647192 hasConcept C165901193 @default.
- W72647192 hasConcept C203014093 @default.
- W72647192 hasConcept C22744801 @default.
- W72647192 hasConcept C2775848585 @default.
- W72647192 hasConcept C2776158911 @default.
- W72647192 hasConcept C2777629521 @default.
- W72647192 hasConcept C2778672586 @default.
- W72647192 hasConcept C40767141 @default.
- W72647192 hasConcept C54355233 @default.
- W72647192 hasConcept C547475151 @default.
- W72647192 hasConcept C86803240 @default.
- W72647192 hasConcept C89423630 @default.
- W72647192 hasConceptScore W72647192C104317684 @default.
- W72647192 hasConceptScore W72647192C150194340 @default.
- W72647192 hasConceptScore W72647192C153911025 @default.
- W72647192 hasConceptScore W72647192C165901193 @default.
- W72647192 hasConceptScore W72647192C203014093 @default.
- W72647192 hasConceptScore W72647192C22744801 @default.
- W72647192 hasConceptScore W72647192C2775848585 @default.
- W72647192 hasConceptScore W72647192C2776158911 @default.
- W72647192 hasConceptScore W72647192C2777629521 @default.
- W72647192 hasConceptScore W72647192C2778672586 @default.
- W72647192 hasConceptScore W72647192C40767141 @default.
- W72647192 hasConceptScore W72647192C54355233 @default.
- W72647192 hasConceptScore W72647192C547475151 @default.
- W72647192 hasConceptScore W72647192C86803240 @default.
- W72647192 hasConceptScore W72647192C89423630 @default.
- W72647192 hasIssue "4" @default.
- W72647192 hasLocation W726471921 @default.
- W72647192 hasOpenAccess W72647192 @default.
- W72647192 hasPrimaryLocation W726471921 @default.
- W72647192 hasRelatedWork W2027752418 @default.
- W72647192 hasRelatedWork W2056542628 @default.
- W72647192 hasRelatedWork W2060580415 @default.
- W72647192 hasRelatedWork W2067449492 @default.
- W72647192 hasRelatedWork W2087908294 @default.
- W72647192 hasRelatedWork W2143650657 @default.
- W72647192 hasRelatedWork W2147662622 @default.
- W72647192 hasRelatedWork W2168023518 @default.
- W72647192 hasRelatedWork W2365662586 @default.
- W72647192 hasRelatedWork W2374676773 @default.
- W72647192 hasRelatedWork W2559963120 @default.
- W72647192 hasRelatedWork W2567496223 @default.
- W72647192 hasRelatedWork W2617387649 @default.
- W72647192 hasRelatedWork W2181310702 @default.
- W72647192 hasVolume "2" @default.
- W72647192 isParatext "false" @default.
- W72647192 isRetracted "false" @default.
- W72647192 magId "72647192" @default.
- W72647192 workType "article" @default.