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- W7268420 abstract "MMPs play a crucial role in the process of cancer invasion and metastasis.The influence of NAC on invasion and MMP-9 production of human bladder cancer cell line T24 was investigated using an in vitro invasion assay, gelatin zymography, Western and Northern blot analyses and RT-PCR assays.TPA increased the number of invading T24 cells through reconstituted basement membrane more than 10-fold compared to basal condition. NAC inhibited TPA-enhanced invasion dose-dependently. TPA increased the MMP-9 production by T24 cells without altering expression of TIMP-1 gene, while NAC suppressed TPA-enhanced production of MMP-9. Neither TPA nor NAC altered TIMP-1 mRNA level in T24 cells. In vitro experiments demonstrated that MMP-9 was directly inhibited by NAC but was not influenced by TPA.NAC limits invasion of T24 human bladder cancer cells by inhibiting the MMP-9 production in addition to a direct inhibition of MMP-9 activity." @default.
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- W7268420 date "2001-04-13" @default.
- W7268420 modified "2023-09-23" @default.
- W7268420 title "Inhibitory effect of N-acetylcysteine on invasion and MMP-9 production of T24 human bladder cancer cells." @default.
- W7268420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11299737" @default.
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