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- W73026478 abstract "This chapter describes accurate determination of molecular mass for proteins isolated from polyacrylamide gels. The molecular mass (MM) of a protein provides important information for identification and purification. SDS-PAGE is commonly used to determine protein MM values because of its simplicity and wide applicability. However, the accuracy of MM values determined from SDS-PAGE is limited because they are a function of relative mobility, which is subject to many experimental variables. This is especially true for small (>10 kD) proteins. This chapter describes a method to remove proteins from SDS gels for analysis by time-of-flight MS. Initial efforts were directed toward sample preparation for PDMS. The sample preparation method was extended to matrix-assisted laser desorption MS (MALDI). PDMS gave accurate MM values for peptides and small proteins, and was reasonably insensitive to gel components such as SDS and dyes. The efforts were then extended to MALDI, which indicated that it was more sensitive and accurate than PDMS for small proteins. However, MALDI analysis of larger proteins often resulted in poor quality spectra." @default.
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- W73026478 date "1993-01-01" @default.
- W73026478 modified "2023-09-26" @default.
- W73026478 title "ACCURATE DETERMINATION OF MOLECULAR MASS FOR PROTEINS ISOLATED FROM POLYACRYLAMIDE GELS" @default.
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- W73026478 doi "https://doi.org/10.1016/b978-0-12-058757-5.50016-0" @default.
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