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- W73245715 abstract "Electrophoretic analysis of enzymes from tropical plants can be very diffie-ult due to thepresence of high concentrations of phenolics in plant tissues. It is believed that these high tannin levelsmay serve to protect plants from the numerous insect predators present in the tropics. Differentmethods of extraction of enzymes prior to electrophoresis are suggested. A number of factors whichmake plant proteins practically unstable and difficult to work with are also mentioned. The occurrenceof tannins in plants and the reactions of these phenolic compounds with proteins and enzymesare discussed. Techniques for the extraction of enzymes from tannin-rich plants should involve theremoval of phenolic substrates, the inhibition of O-dlfthenol-oxidase activity or the removal ofquinones formed from oxidation by reducing the quinones back to the O-dlfthenol. Chemicals addedinto the extracting medium to overcome the problem of interference by tannins include polymers,copper chelating agents, thiols, ascorbate, metabisulphite, dithionite and b01!ine serum albumin." @default.
- W73245715 created "2016-06-24" @default.
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- W73245715 date "1985-01-01" @default.
- W73245715 modified "2023-09-23" @default.
- W73245715 title "Preparation of Extracts from Tropical Plants for Electrophoresis — An Analysis of Methodology" @default.
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