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- W73253004 abstract "It has been proposed that phages can be used commercially as a biopesticide forthe control of fire blight caused by the phytopathogen Erwinia amylovora. The aim ofthese studies was to investigate two common bacterial resistance mechanisms, lysogenyand exopolysaccharide production and their influence on phage pathogenesis. A multiplexreal-time PCR protocol was designed to monitor and quantify Podoviridae andMyoviridae phages. This protocol is compatible with known E. amylovora and Pantoeaagglomerans rtPCR primers/probes which allowed simultaneous study of both phage andbacterial targets. Using in vitro positive phage selection, bacteriophage insensitivederivatives were isolated within sensitive populations of E. amylovora. Prophagescreening with real-time PCR and mitomycin C induction determined that the insensitivederivatives harboured the temperate Podoviridae phage ΦEaTlOO. Lysogenic conversionresulted in resistance to secondary homologous phage infections. Prophage screening ofenvironmental samples of E. amylovora and P. agglomerans collected from variouslocations in Canada, United States and Europe did not demonstrate lysogeny. Therefore,lysogeny is rare or absent while these bacterial species reside on the plant.Recombineering was used to construct exopolysaccharide deficient E. amylovoramutants. The EPS amylovoran mutants became resistant to Podoviridae and certainSiphoviridae phages. Increasing amylovoran production increased phage populationgrowth, presumably by increasing the total number of bacterial cell surface receptorswhich promoted increased phage infections. In contrast, amylovoran did not playa role inMyoviridae infections, nor did production of the EPS levan for any phage pathogenesis." @default.
- W73253004 created "2016-06-24" @default.
- W73253004 creator A5089913383 @default.
- W73253004 date "2012-04-04" @default.
- W73253004 modified "2023-09-23" @default.
- W73253004 title "Erwinia amylovora bacteriophage resistance" @default.
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