Matches in SemOpenAlex for { <https://semopenalex.org/work/W73347686> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W73347686 abstract "Background: Most clinical studies used autologous bone marrow stem cells (BMSCs) for myocardial repair in elderly patients. Accounting for the limited success in clinical studies, we hypothesized that aging impairs the reparability of BMSCs which limits their therapeutic efficacy. Methods and Results : We compared MSCs from young ( Yng MSCs; 8–12 weeks) and old ( Old MSCs; 24–26 weeks) rats for their responsiveness to anoxia and therapeutic potential. A significantly higher expression of angiogenic growth factors was observed by Yng MSCs under anoxia as compared with Old MSCs, cultured either alone or in co-culture ( Co-old MSCs) with Yng MSCs. Likewise, Yng MSCs showed significantly higher ability to form tubular structures during Matrigel assay. LDH release assay showed that Yng MSCs were more tolerant to apoptotic stimuli (glucose and serum starvation under anoxia) as compared with Old MSCs and Co-old MSCs, with a possible role for pro-survival proteins p21 and p27 which showed increased gene expression (>8-fold and 13-fold respectively) in Yng MSCs under anoxia as compared with Old MSCs. For in vivo studies, acute myocardial infarction model was developed in Fisher-344 rats (n=38). The animals were grouped to receive 70μl DMEM without cells (group-1) or containing 3×10 6 Yng MSCs (PKH67 labeled; group-2), or Old MSCs (PKH26 labeled; group-3) and mixture of Yng MSCs+ Old MSCs (1.5×10 6 cells each, group-4). Histological studies revealed that by day-7, Yng MSCs were already showing elongated morphology with orientation similar to the host muscle architecture. Transmission electron microscopy and confocal imaging after immuno-staining for vonWillebrand Factor-VIII, myosin heavy chain, cardiac actinin and connexin-43, showed superior angiomyogenic potential of Yng MSCs. Echocardiography showed significantly improved indices of left ventricle contractile function in group-4. Conclusions: Aging significantly weakened the responsiveness of Old MSCs to anoxia and their differentiation potential. Their reparability of infarcted heart was also impaired with aging which was not improved by the presence of Yng MSCs. Hence, transplantation of stem cells from young donors should be considered for heart cell therapy in elderly patients for better prognosis." @default.
- W73347686 created "2016-06-24" @default.
- W73347686 creator A5032353933 @default.
- W73347686 creator A5043017215 @default.
- W73347686 creator A5043760565 @default.
- W73347686 creator A5049157795 @default.
- W73347686 creator A5056110348 @default.
- W73347686 creator A5058571380 @default.
- W73347686 date "2007-10-16" @default.
- W73347686 modified "2023-09-23" @default.
- W73347686 title "Abstract 1280: Transcriptional Profiling Of Young And Old Mesenchymal Stem Cells For Their Response To Oxygen Deprivation And Myocardial Reparability" @default.
- W73347686 doi "https://doi.org/10.1161/circ.116.suppl_16.ii_261-b" @default.
- W73347686 hasPublicationYear "2007" @default.
- W73347686 type Work @default.
- W73347686 sameAs 73347686 @default.
- W73347686 citedByCount "0" @default.
- W73347686 crossrefType "journal-article" @default.
- W73347686 hasAuthorship W73347686A5032353933 @default.
- W73347686 hasAuthorship W73347686A5043017215 @default.
- W73347686 hasAuthorship W73347686A5043760565 @default.
- W73347686 hasAuthorship W73347686A5049157795 @default.
- W73347686 hasAuthorship W73347686A5056110348 @default.
- W73347686 hasAuthorship W73347686A5058571380 @default.
- W73347686 hasConcept C104317684 @default.
- W73347686 hasConcept C126322002 @default.
- W73347686 hasConcept C127561419 @default.
- W73347686 hasConcept C142724271 @default.
- W73347686 hasConcept C150903083 @default.
- W73347686 hasConcept C16685009 @default.
- W73347686 hasConcept C190283241 @default.
- W73347686 hasConcept C198826908 @default.
- W73347686 hasConcept C207001950 @default.
- W73347686 hasConcept C2778608917 @default.
- W73347686 hasConcept C2780007613 @default.
- W73347686 hasConcept C2780394083 @default.
- W73347686 hasConcept C500558357 @default.
- W73347686 hasConcept C502942594 @default.
- W73347686 hasConcept C55493867 @default.
- W73347686 hasConcept C71924100 @default.
- W73347686 hasConcept C86803240 @default.
- W73347686 hasConceptScore W73347686C104317684 @default.
- W73347686 hasConceptScore W73347686C126322002 @default.
- W73347686 hasConceptScore W73347686C127561419 @default.
- W73347686 hasConceptScore W73347686C142724271 @default.
- W73347686 hasConceptScore W73347686C150903083 @default.
- W73347686 hasConceptScore W73347686C16685009 @default.
- W73347686 hasConceptScore W73347686C190283241 @default.
- W73347686 hasConceptScore W73347686C198826908 @default.
- W73347686 hasConceptScore W73347686C207001950 @default.
- W73347686 hasConceptScore W73347686C2778608917 @default.
- W73347686 hasConceptScore W73347686C2780007613 @default.
- W73347686 hasConceptScore W73347686C2780394083 @default.
- W73347686 hasConceptScore W73347686C500558357 @default.
- W73347686 hasConceptScore W73347686C502942594 @default.
- W73347686 hasConceptScore W73347686C55493867 @default.
- W73347686 hasConceptScore W73347686C71924100 @default.
- W73347686 hasConceptScore W73347686C86803240 @default.
- W73347686 hasIssue "suppl_16" @default.
- W73347686 hasLocation W733476861 @default.
- W73347686 hasOpenAccess W73347686 @default.
- W73347686 hasPrimaryLocation W733476861 @default.
- W73347686 hasRelatedWork W2070997638 @default.
- W73347686 hasRelatedWork W2074910936 @default.
- W73347686 hasRelatedWork W2120735623 @default.
- W73347686 hasRelatedWork W2316107365 @default.
- W73347686 hasRelatedWork W2367140913 @default.
- W73347686 hasRelatedWork W2377483921 @default.
- W73347686 hasRelatedWork W2383151259 @default.
- W73347686 hasRelatedWork W2415550550 @default.
- W73347686 hasRelatedWork W4253073611 @default.
- W73347686 hasRelatedWork W4255572155 @default.
- W73347686 hasVolume "116" @default.
- W73347686 isParatext "false" @default.
- W73347686 isRetracted "false" @default.
- W73347686 magId "73347686" @default.
- W73347686 workType "article" @default.