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- W7335412 abstract "Objective To develop an in vitro culture system for Cryptosporidium parvum in Madin-Darby canine kidney (MDCK) cell and observe its life cycle (from desquamate to oocyst). Methods Oocysts of C. parvum were co-cultured with MDCK cells in vitro. Culture condition was optimized and the life cycle of C.parvum investigated. Results The optimal culture conditions for C. parvum in MDCK cells were 2.0 x 10(5) cells cultured for 12 h, and infected by 1.0 x 10(5) oocysts in the Dulbecco's Modified Eagle Medium with 5% FBS. Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72nd hour, many oocysts emerged. Inoculated by the C. parvum-infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected. Conclusion The culture system provides a model for propagation of the parasites and demonstrates a complete in vitro life cycle of C. parvum." @default.
- W7335412 created "2016-06-24" @default.
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- W7335412 date "2009-06-01" @default.
- W7335412 modified "2023-09-28" @default.
- W7335412 title "In vitro cultivation model of Cryptosporidium parvum in MDCK cells and its development" @default.
- W7335412 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19852362" @default.
- W7335412 hasPublicationYear "2009" @default.
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