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• W73360605 abstract "The contribution of adenovirus early genes, other than that of the well-documented EIa immediate early gene, to the transcriptional regulation of the viral EIIa early transcription unit was examined. HeLa cells were transfected with EIIa-containing plasmids and co-transfected with distinct plasmids bearing one of the viral regions EIa, EIII or EIV. Co-transfection with the EIV-recombinants, but not the EIII constructs, stimulated specific transcription from the major EIIaE start site (EIIaE1) by 5- to 15-fold, as concluded from quantitative S1 nuclease analysis of cytoplasmic RNA and in vitro nuclear 'run-on' transcription assays. The extent of the EIV-induced stimulation was similar to that achieved by EIa under identical conditions. However, in contrast to our observations for EIa-mediated stimulation, where no unique EIIaE1 promoter elements were implicated, maximal induction by EIV requires sequences between positions -48 and -19 (with respect to the EIIaE1 start site)." @default.
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• W73360605 date "1985-06-01" @default.
• W73360605 modified "2023-10-18" @default.
• W73360605 title "Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit." @default.
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• W73360605 doi "https://doi.org/10.1002/j.1460-2075.1985.tb03812.x" @default.
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