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• W735325 endingPage "150" @default.
• W735325 startingPage "139" @default.
• W735325 abstract "We describe in situ hybridization protocols using peptide nucleic acid (PNA) as a probe for detecting HIV-1 DNA in virus-infected cells and the subsequent detection of cellular and/or viral proteins. Because a PNA probe of approx 20 bases was sufficiently long to detect a specific target sequence, a conserved sequence of such a short length was easily identified. Therefore, this probe is valuable even to identify quasi-species of HIV-1. In addition, we adopted a catalyzed signal amplification method to amplify weak viral DNA signals; thus, stringent washing was crucial for eliminating false-positive signals. Our double-staining method using PNA-in situ hybridization and subsequent immunostaining enabled the active and inactive proviruses to be distinguished." @default.
• W735325 created "2016-06-24" @default.
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• W735325 date "2006-06-30" @default.
• W735325 modified "2023-09-26" @default.
• W735325 title "PNA-<i>In Situ</i> Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins" @default.
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• W735325 doi "https://doi.org/10.1385/1-59745-007-3:139" @default.
• W735325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16780198" @default.
• W735325 hasPublicationYear "2006" @default.
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