Matches in SemOpenAlex for { <https://semopenalex.org/work/W73605514> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W73605514 endingPage "436" @default.
- W73605514 startingPage "432" @default.
- W73605514 abstract "We report here the development of a rapid and quantitative method for measuring in vitro T cell transformation by human T cell leukemia viruses type I (HTLV-I) and type II (HTLV-II). This method is based on our finding that cocultivation of lethally irradiated HTLV-producing cells with peripheral blood lymphocytes (PBLs) preactivated for 24 hours with phytohemagglutinin and interleukin-2 (IL-2) induces colony formation in methylcellulose-containing medium. Colonies of about 200 cells can be clearly distinguished from background aggregates within four to six days after cocultivation. These colonies gradually increase in size and reach 300 to 1,000 cells within 14 days after cocultivation. Cells of these colonies were infected, as evidenced by expression of viral p19 antigen and the presence of HTLV proviral sequences. These cells proved to be transformed in terms of IL-2- independent continuous growth in liquid medium. Colony formation was found to depend in a linear fashion upon the percentage of the infected cells present in the irradiated cell population and is sufficiently sensitive to detect as few as 1% of virus-producing cells." @default.
- W73605514 created "2016-06-24" @default.
- W73605514 creator A5023295685 @default.
- W73605514 creator A5041759190 @default.
- W73605514 creator A5074987361 @default.
- W73605514 creator A5077300234 @default.
- W73605514 creator A5087590026 @default.
- W73605514 date "1987-08-01" @default.
- W73605514 modified "2023-10-01" @default.
- W73605514 title "A colony assay for in vitro transformation by human T cell leukemia viruses type I and type II" @default.
- W73605514 cites W1970634297 @default.
- W73605514 cites W1979637191 @default.
- W73605514 cites W1980744027 @default.
- W73605514 cites W1990337042 @default.
- W73605514 cites W1991049176 @default.
- W73605514 cites W2004149547 @default.
- W73605514 cites W2015272984 @default.
- W73605514 cites W2022157348 @default.
- W73605514 cites W2032716813 @default.
- W73605514 cites W2034650432 @default.
- W73605514 cites W2047814541 @default.
- W73605514 cites W2048022927 @default.
- W73605514 cites W2060880451 @default.
- W73605514 cites W2061769726 @default.
- W73605514 cites W2067287812 @default.
- W73605514 cites W2083577304 @default.
- W73605514 cites W2094141681 @default.
- W73605514 cites W2095383081 @default.
- W73605514 cites W2105209860 @default.
- W73605514 cites W2123679031 @default.
- W73605514 cites W2156120935 @default.
- W73605514 cites W2180795626 @default.
- W73605514 cites W2400580090 @default.
- W73605514 cites W2400853250 @default.
- W73605514 cites W2414957462 @default.
- W73605514 cites W2427208484 @default.
- W73605514 doi "https://doi.org/10.1182/blood.v70.2.432.bloodjournal702432" @default.
- W73605514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2886161" @default.
- W73605514 hasPublicationYear "1987" @default.
- W73605514 type Work @default.
- W73605514 sameAs 73605514 @default.
- W73605514 citedByCount "9" @default.
- W73605514 countsByYear W736055142017 @default.
- W73605514 crossrefType "journal-article" @default.
- W73605514 hasAuthorship W73605514A5023295685 @default.
- W73605514 hasAuthorship W73605514A5041759190 @default.
- W73605514 hasAuthorship W73605514A5074987361 @default.
- W73605514 hasAuthorship W73605514A5077300234 @default.
- W73605514 hasAuthorship W73605514A5087590026 @default.
- W73605514 hasBestOaLocation W736055141 @default.
- W73605514 hasConcept C104317684 @default.
- W73605514 hasConcept C147483822 @default.
- W73605514 hasConcept C153911025 @default.
- W73605514 hasConcept C159047783 @default.
- W73605514 hasConcept C202751555 @default.
- W73605514 hasConcept C203014093 @default.
- W73605514 hasConcept C204241405 @default.
- W73605514 hasConcept C2777371288 @default.
- W73605514 hasConcept C2778461978 @default.
- W73605514 hasConcept C2908647359 @default.
- W73605514 hasConcept C54355233 @default.
- W73605514 hasConcept C71924100 @default.
- W73605514 hasConcept C86803240 @default.
- W73605514 hasConcept C99454951 @default.
- W73605514 hasConceptScore W73605514C104317684 @default.
- W73605514 hasConceptScore W73605514C147483822 @default.
- W73605514 hasConceptScore W73605514C153911025 @default.
- W73605514 hasConceptScore W73605514C159047783 @default.
- W73605514 hasConceptScore W73605514C202751555 @default.
- W73605514 hasConceptScore W73605514C203014093 @default.
- W73605514 hasConceptScore W73605514C204241405 @default.
- W73605514 hasConceptScore W73605514C2777371288 @default.
- W73605514 hasConceptScore W73605514C2778461978 @default.
- W73605514 hasConceptScore W73605514C2908647359 @default.
- W73605514 hasConceptScore W73605514C54355233 @default.
- W73605514 hasConceptScore W73605514C71924100 @default.
- W73605514 hasConceptScore W73605514C86803240 @default.
- W73605514 hasConceptScore W73605514C99454951 @default.
- W73605514 hasIssue "2" @default.
- W73605514 hasLocation W736055141 @default.
- W73605514 hasOpenAccess W73605514 @default.
- W73605514 hasPrimaryLocation W736055141 @default.
- W73605514 hasRelatedWork W1487857142 @default.
- W73605514 hasRelatedWork W1702322095 @default.
- W73605514 hasRelatedWork W1865764402 @default.
- W73605514 hasRelatedWork W1975114493 @default.
- W73605514 hasRelatedWork W2031928526 @default.
- W73605514 hasRelatedWork W2118087741 @default.
- W73605514 hasRelatedWork W2139448775 @default.
- W73605514 hasRelatedWork W2156948268 @default.
- W73605514 hasRelatedWork W3029386773 @default.
- W73605514 hasRelatedWork W4300420468 @default.
- W73605514 hasVolume "70" @default.
- W73605514 isParatext "false" @default.
- W73605514 isRetracted "false" @default.
- W73605514 magId "73605514" @default.
- W73605514 workType "article" @default.