Matches in SemOpenAlex for { <https://semopenalex.org/work/W73638324> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W73638324 abstract "SUSANA WIDJAJA. Virological and Immunological Studies of Dengue Virus Infection in Pigtail Macaques (Macaca nemestrina). Supervised by DONDIN SAJUTHI, JOKO PAMUNGKAS, DIAH ISKANDRIATI, and PATRICK J BLAIR. A non-human primate (NHP) model is essential for the study of dengue hemorrhagic fever (DHF) pathogenesis and the evaluation of dengue (DEN) vaccine and antiviral drug. Until now, it has been difficult to find an NHP DHF pathogenesis model. Therefore, an evaluation of a DEN vaccine candidate is performed in NHPs that show viremia after infected by DEN virus and the vaccine efficacy is its capability to develop immunity that reduces viremia when vaccinated NHPs are challenged by DEN virus. In this study, the potential of pigtail macaque to serve as an animal model for DEN vaccine testing was evaluated. Homologous sequential DEN challenges were conducted using primary viral isolates from DEN patients in Indonesia. Two parameters, the ability to support dengue viremia and to produce sufficient antibody responses were measured. This study shows that primary infections of all four DEN serotypes cause consistent, measurable viremia in pigtail macaques. The responses of IgM, IgG and avidity antibody following primary and secondary DEN infections are similar with antibody responses in human. The immunity produced by primary infection is sufficient to protect against homologous virus. This species of macaque therefore appears to be a suitable alternative model for testing DEN vaccine candidates. Besides antibody, T lymphocyte also has an important role in the protection and pathogenesis of DEN diseases. DEN specific T lymphocyte measurements, ELISPOT and intracellular cytokine staining-flow cytometry (ICFC), were developed to support DEN studies in pigtail macaque. Peripheral blood mononuclear cells (PBMC) collected before and after DEN infections were tested. ELISPOT results show increase of DEN specific interferon-γ (IFN-γ) producing cells as an individual response of pigtail to primary DEN-1, DEN-3 or DEN-4 infections. Using pools of PBMC taken from several animals, ELISPOT and intracellular cytokine staining-flow cytometry (IC-FC) was run side by side to quantify DEN specific lymphocytes following primary and secondary DEN-2 infections. ELISPOT revealed an increase of DEN specific IFN-γ producing cells following primary infection and a significant increase after secondary infection. Similarly, IC-FC also measured an increase of DEN specific producing IFN-γ CD3+CD4+ and CD3+CD4T lymphocytes. As such, ELISPOT and IC-FC can be applied to measure DEN specific T lymphocytes in pigtail macaques. Therefore, the application of these assays would be useful in elaborating adaptive immunity induced by vaccine and the level of protection. Furthermore, the development of pigtail as DHF model can be evaluated when further research on the cross-reactive T lymphocyte and antibody responses during secondary heterologous is conducted." @default.
- W73638324 created "2016-06-24" @default.
- W73638324 creator A5001819517 @default.
- W73638324 date "2010-01-01" @default.
- W73638324 modified "2023-09-23" @default.
- W73638324 title "Virological and Immunological Studies of Dengue Virus Infection in Pigtail Macaques (Macaca nemestrina)." @default.
- W73638324 cites W1513149807 @default.
- W73638324 cites W1557590746 @default.
- W73638324 cites W1567194265 @default.
- W73638324 cites W1571993997 @default.
- W73638324 cites W171675974 @default.
- W73638324 cites W183775175 @default.
- W73638324 cites W1969021764 @default.
- W73638324 cites W2018466688 @default.
- W73638324 cites W2032676385 @default.
- W73638324 cites W2040552041 @default.
- W73638324 cites W2043271242 @default.
- W73638324 cites W2044123818 @default.
- W73638324 cites W2049406258 @default.
- W73638324 cites W2063994632 @default.
- W73638324 cites W2072041855 @default.
- W73638324 cites W2076919706 @default.
- W73638324 cites W2092074834 @default.
- W73638324 cites W2096077114 @default.
- W73638324 cites W2100371398 @default.
- W73638324 cites W2113422043 @default.
- W73638324 cites W2118871112 @default.
- W73638324 cites W2129245146 @default.
- W73638324 cites W2130592278 @default.
- W73638324 cites W2140167271 @default.
- W73638324 cites W2163600402 @default.
- W73638324 cites W2169873843 @default.
- W73638324 hasPublicationYear "2010" @default.
- W73638324 type Work @default.
- W73638324 sameAs 73638324 @default.
- W73638324 citedByCount "2" @default.
- W73638324 countsByYear W736383242013 @default.
- W73638324 crossrefType "journal-article" @default.
- W73638324 hasAuthorship W73638324A5001819517 @default.
- W73638324 hasConcept C151730666 @default.
- W73638324 hasConcept C159047783 @default.
- W73638324 hasConcept C159654299 @default.
- W73638324 hasConcept C196594336 @default.
- W73638324 hasConcept C203014093 @default.
- W73638324 hasConcept C2522874641 @default.
- W73638324 hasConcept C2776185481 @default.
- W73638324 hasConcept C2778950215 @default.
- W73638324 hasConcept C2779341262 @default.
- W73638324 hasConcept C2779635636 @default.
- W73638324 hasConcept C533803919 @default.
- W73638324 hasConcept C86803240 @default.
- W73638324 hasConcept C8891405 @default.
- W73638324 hasConceptScore W73638324C151730666 @default.
- W73638324 hasConceptScore W73638324C159047783 @default.
- W73638324 hasConceptScore W73638324C159654299 @default.
- W73638324 hasConceptScore W73638324C196594336 @default.
- W73638324 hasConceptScore W73638324C203014093 @default.
- W73638324 hasConceptScore W73638324C2522874641 @default.
- W73638324 hasConceptScore W73638324C2776185481 @default.
- W73638324 hasConceptScore W73638324C2778950215 @default.
- W73638324 hasConceptScore W73638324C2779341262 @default.
- W73638324 hasConceptScore W73638324C2779635636 @default.
- W73638324 hasConceptScore W73638324C533803919 @default.
- W73638324 hasConceptScore W73638324C86803240 @default.
- W73638324 hasConceptScore W73638324C8891405 @default.
- W73638324 hasLocation W736383241 @default.
- W73638324 hasOpenAccess W73638324 @default.
- W73638324 hasPrimaryLocation W736383241 @default.
- W73638324 hasRelatedWork W1964123514 @default.
- W73638324 hasRelatedWork W1984311170 @default.
- W73638324 hasRelatedWork W1984329853 @default.
- W73638324 hasRelatedWork W2024851127 @default.
- W73638324 hasRelatedWork W2043176595 @default.
- W73638324 hasRelatedWork W2093427826 @default.
- W73638324 hasRelatedWork W2127635055 @default.
- W73638324 hasRelatedWork W2130034923 @default.
- W73638324 hasRelatedWork W2130978869 @default.
- W73638324 hasRelatedWork W2137824026 @default.
- W73638324 hasRelatedWork W2144780961 @default.
- W73638324 hasRelatedWork W2147694159 @default.
- W73638324 hasRelatedWork W2149762607 @default.
- W73638324 hasRelatedWork W2160836632 @default.
- W73638324 hasRelatedWork W2166867894 @default.
- W73638324 hasRelatedWork W2168885953 @default.
- W73638324 hasRelatedWork W2189914655 @default.
- W73638324 hasRelatedWork W2590701925 @default.
- W73638324 hasRelatedWork W2803064139 @default.
- W73638324 hasRelatedWork W3079751428 @default.
- W73638324 isParatext "false" @default.
- W73638324 isRetracted "false" @default.
- W73638324 magId "73638324" @default.
- W73638324 workType "article" @default.