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- W73671936 abstract "Publisher Summary Scanning electron microscopy (SEM) is advantageous in the morphological research of intracellular structures that are spatially arranged intricatly. Advances in instruments and specimen preparation techniques permit intracellular fine structures to be studied by SEM. This chapter illustrates the intracellular structures observed by SEM. The specimens are prepared by the O–D–O method, the aldehyde-prefix O–D–O method, or the rapid freezing or freeze substitution O–D–O method. SEM appears appropriate for studying the spatially complicated structures of the Golgi complex. SEM is better able to reveal the whole external form of mitochondria than is TEM, which tends to give a misleading impression of the form. This is because a thin section presents only one profile of the mitochondrion, which depends upon the orientation of the organelle in relation to the plane of the section. The external form viewed by SEM shows extremely variable shapes. Although spherical, ellipsoid, and club-like shapes are most common, irregular shapes are also observed in many kinds of cells." @default.
- W73671936 created "2016-06-24" @default.
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- W73671936 date "1987-01-01" @default.
- W73671936 modified "2023-10-18" @default.
- W73671936 title "Eukaryotes: Scanning Electron Microscopy of Intracellular Structures" @default.
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- W73671936 doi "https://doi.org/10.1016/b978-0-08-091882-2.50007-5" @default.
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