FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W73875906> ?p ?o ?g. }

• W73875906 endingPage "60" @default.
• W73875906 startingPage "1056" @default.
• W73875906 abstract "Myoglobin is expressed exclusively in striated skeletal muscles and has been implicated in nitric oxide scavenging. Accumulating data suggest a critical role for nitric oxide in both the endogenous and therapeutic angiogenic response to ischemia. A clear role for myoglobin in ischemic skeletal muscle is uncertain. We hypothesized that myoglobin overexpression has an adverse impact on the angiogenic response to ischemia.Muscle-specific myoglobin over-expressing transgenic mice (MbTG, n = 11), wild type littermates (WT, n = 23) underwent unilateral femoral artery ligation and excision. Laser doppler perfusion imaging was used to monitor changes in hindlimb perfusion before surgery and weekly after surgery up to 28 days. Tissue ischemia was assessed by a necrosis incidence. Upon termination of the experiment (28 days after surgery), skeletal muscles (gastrocnemius, and tibialis anterior) were harvested, the distal part of the muscle was frozen and embedded for histology study, the proximal part was used either to detect vascular endothelial growth factor (VEGF) level with enzyme-linked immunosorbent assays (ELISA) or to determine the proliferation (proliferating cell nuclear antigen (PCNA)) and apoptosis (Bax, and Bcl-2) condition in ischemic muscle by Western blotting. Capillaries were stained with endothelial phosphate alkaline staining and vascular density was expressed in capillaries/fiber.The recovery of perfusion in MbTG mice was similar to that of WT mice on day 7 (0.485 +/- 0.095 vs 0.500 +/- 0.084) but was significantly less on day 14 (0.536 +/- 0.086 vs 0.623 +/- 0.077, P < 0.05), day 21 (0.588 +/- 0.082 vs 0.684 +/- 0.068, P < 0.01) and day 28 (0.606 +/- 0.079 vs 0.733 +/- 0.093, P < 0.01). The necrosis incidence was higher in MbTG than in WT (54.5% vs 21.6%). Vascular density was less in MbTG compared with that in WT (gastrocnemius 0.19 +/- 0.08 vs 0.30 +/- 0.08, P < 0.05; tibialis anterior 0.22 +/- 0.11 vs 0.33 +/- 0.04, P < 0.05). With ischemic injury, the VEGF level was increased in both MbTG and WT (45.2% and 20.4%, respectively). Western blotting showed that after hindlimb ischemia the proliferation was similar in both MbTG and WT, however, apoptosis was increased in MbTG relative to WT, shown as more expression of Bax and less expression of Bcl-2.An increase in expression of myoglobin protein in skeletal muscle reduces the endogenous perfusion recovery following surgically induced hind-limb ischemia." @default.
• W73875906 created "2016-06-24" @default.
• W73875906 creator A5032271174 @default.
• W73875906 creator A5036640081 @default.
• W73875906 creator A5049821226 @default.
• W73875906 creator A5056292287 @default.
• W73875906 date "2009-05-05" @default.
• W73875906 modified "2023-09-26" @default.
• W73875906 title "Myoglobin over-expression attenuates angiogenic response in hindlimb ischemia in mice." @default.
• W73875906 cites W161287260 @default.
• W73875906 cites W1976090430 @default.
• W73875906 cites W1981140090 @default.
• W73875906 cites W2016594382 @default.
• W73875906 cites W2030351692 @default.
• W73875906 cites W2038369760 @default.
• W73875906 cites W2066538437 @default.
• W73875906 cites W2087221897 @default.
• W73875906 cites W2100425244 @default.
• W73875906 cites W2118408607 @default.
• W73875906 cites W2143260418 @default.
• W73875906 cites W2163926528 @default.
• W73875906 cites W232407047 @default.
• W73875906 cites W2324677651 @default.
• W73875906 cites W2413727280 @default.
• W73875906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19493440" @default.
• W73875906 hasPublicationYear "2009" @default.
• W73875906 type Work @default.
• W73875906 sameAs 73875906 @default.
• W73875906 citedByCount "2" @default.
• W73875906 countsByYear W738759062014 @default.
• W73875906 countsByYear W738759062015 @default.
• W73875906 crossrefType "journal-article" @default.
• W73875906 hasAuthorship W73875906A5032271174 @default.
• W73875906 hasAuthorship W73875906A5036640081 @default.
• W73875906 hasAuthorship W73875906A5049821226 @default.
• W73875906 hasAuthorship W73875906A5056292287 @default.
• W73875906 hasConcept C126322002 @default.
• W73875906 hasConcept C134018914 @default.
• W73875906 hasConcept C142724271 @default.
• W73875906 hasConcept C146957229 @default.
• W73875906 hasConcept C167734588 @default.
• W73875906 hasConcept C185592680 @default.
• W73875906 hasConcept C2775872116 @default.
• W73875906 hasConcept C2777025900 @default.
• W73875906 hasConcept C2778063876 @default.
• W73875906 hasConcept C2779959927 @default.
• W73875906 hasConcept C503630168 @default.
• W73875906 hasConcept C51738704 @default.
• W73875906 hasConcept C519581460 @default.
• W73875906 hasConcept C541997718 @default.
• W73875906 hasConcept C55493867 @default.
• W73875906 hasConcept C71924100 @default.
• W73875906 hasConceptScore W73875906C126322002 @default.
• W73875906 hasConceptScore W73875906C134018914 @default.
• W73875906 hasConceptScore W73875906C142724271 @default.
• W73875906 hasConceptScore W73875906C146957229 @default.
• W73875906 hasConceptScore W73875906C167734588 @default.
• W73875906 hasConceptScore W73875906C185592680 @default.
• W73875906 hasConceptScore W73875906C2775872116 @default.
• W73875906 hasConceptScore W73875906C2777025900 @default.
• W73875906 hasConceptScore W73875906C2778063876 @default.
• W73875906 hasConceptScore W73875906C2779959927 @default.
• W73875906 hasConceptScore W73875906C503630168 @default.
• W73875906 hasConceptScore W73875906C51738704 @default.
• W73875906 hasConceptScore W73875906C519581460 @default.
• W73875906 hasConceptScore W73875906C541997718 @default.
• W73875906 hasConceptScore W73875906C55493867 @default.
• W73875906 hasConceptScore W73875906C71924100 @default.
• W73875906 hasIssue "9" @default.
• W73875906 hasLocation W738759061 @default.
• W73875906 hasOpenAccess W73875906 @default.
• W73875906 hasPrimaryLocation W738759061 @default.
• W73875906 hasRelatedWork W1963823286 @default.
• W73875906 hasRelatedWork W1982978096 @default.
• W73875906 hasRelatedWork W1987397151 @default.
• W73875906 hasRelatedWork W2015433973 @default.
• W73875906 hasRelatedWork W2016412805 @default.
• W73875906 hasRelatedWork W2028971889 @default.
• W73875906 hasRelatedWork W2038369760 @default.
• W73875906 hasRelatedWork W2071721339 @default.
• W73875906 hasRelatedWork W2082137186 @default.
• W73875906 hasRelatedWork W2104502187 @default.
• W73875906 hasRelatedWork W2170602910 @default.
• W73875906 hasRelatedWork W2276950657 @default.
• W73875906 hasRelatedWork W2336342080 @default.
• W73875906 hasRelatedWork W2355421330 @default.
• W73875906 hasRelatedWork W2380445168 @default.
• W73875906 hasRelatedWork W2390394327 @default.
• W73875906 hasRelatedWork W2589567928 @default.
• W73875906 hasRelatedWork W2604968133 @default.
• W73875906 hasRelatedWork W3030714337 @default.
• W73875906 hasRelatedWork W2740185396 @default.
• W73875906 hasVolume "122" @default.
• W73875906 isParatext "false" @default.
• W73875906 isRetracted "false" @default.
• W73875906 magId "73875906" @default.
• W73875906 workType "article" @default.