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- W73972891 endingPage "1065" @default.
- W73972891 startingPage "1029" @default.
- W73972891 abstract "Genetic manipulation has become widely used in the study of purple photosynthetic bacteria. Some species have been extensively characterized genetically and thus are readily amenable to new applications of these techniques, while in other species genetic work is being developed and may require some adaptation of existing protocols. Genetic studies in purple bacteria include both classical genetic mapping that yields information about the genomic distribution of the genetic loci under study and molecular genetic techniques that employ recombinant DNA techniques and center on analysis at a nucleotide level. As a result of these studies, a large number of genes have been characterized, most prominently those whose products are involved in photosynthesis and nitrogen fixation, but also including a variety of other processes. Techniques that have been used to identify genes range from complementation ofmutant strains to hybridization probes based on homologous genes or the amino acid sequences of proteins. The functional properties of genes have been studied by the generation of random mutations by chemical or transposon mutagenesis and by site-directed insertions, deletions and alterations of nucleotide sequences. The transcription and translation of genes have been investigated through gene fusions and in vitro and heterologous expression systems. The combination of classical genetics with the advances in molecular biological techniques provides an array of tools for dissecting the underlying basis for metabolic processes in these bacteria." @default.
- W73972891 created "2016-06-24" @default.
- W73972891 creator A5084478928 @default.
- W73972891 creator A5067024347 @default.
- W73972891 date "1995-01-01" @default.
- W73972891 modified "2023-10-16" @default.
- W73972891 title "Genetic Manipulation of Purple Photosynthetic Bacteria" @default.
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