FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W74071586> ?p ?o ?g. }

• W74071586 endingPage "3932" @default.
• W74071586 startingPage "3923" @default.
• W74071586 abstract "Research Article1 December 1990free access Agonistic and antagonistic activities of RU486 on the functions of the human progesterone receptor. M. E. Meyer M. E. Meyer Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author A. Pornon A. Pornon Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author J. W. Ji J. W. Ji Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author M. T. Bocquel M. T. Bocquel Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author P. Chambon P. Chambon Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author H. Gronemeyer H. Gronemeyer Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author M. E. Meyer M. E. Meyer Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author A. Pornon A. Pornon Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author J. W. Ji J. W. Ji Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author M. T. Bocquel M. T. Bocquel Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author P. Chambon P. Chambon Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author H. Gronemeyer H. Gronemeyer Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. Search for more papers by this author Author Information M. E. Meyer1, A. Pornon1, J. W. Ji1, M. T. Bocquel1, P. Chambon1 and H. Gronemeyer1 1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France. The EMBO Journal (1990)9:3923-3932https://doi.org/10.1002/j.1460-2075.1990.tb07613.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info RU486 induced the binding to a palindromic progestin responsive element (PRE) in vitro of homo- and heterodimers of the human progesterone receptor (hPR) isoforms A and B, present in T47D breast cancer cells or in HeLa cells transiently expressing the recombinant proteins. The resulting complexes were indistinguishable from those induced with the agonist R5020 with respect to specificity, affinity and stability. Ligand exposure was a necessary prerequisite to observe PR/PRE complexes. Antagonist-induced complexes migrated more rapidly during electrophoresis than agonist-induced ones, and no ‘mixed’ PR/RU486-PR/R5020 complexes were observed, suggesting that the dimerization interfaces of agonist- and antagonist-bound molecules are non-compatible. The analysis of a series of deletion mutants and chimeric receptors revealed the presence of two transcription activation functions (TAFs), located in the N-terminal region A/B (TAF-1) and the hormone binding domain (TAF-2). In the presence of agonists, both TAFs were active in HeLa cells. In the presence of RU486 TAF-2 was inactive, while TAF-1 within the hPR form B/RU486 complex activated transcription from a reporter gene containing a single palindromic PRE. We consider this to be the most convincing evidence that the receptor/RU486-complex does in fact bind to PREs in vivo. No transcriptional activation was observed in the presence of RU486 from a reporter gene containing the complex MMTV-LTR PRE. In contrast to hPR form B, form A was not able to activate transcription from PRE/GRE-tk-CAT in the presence of RU486. In vivo competition between hPR/RU486 and either cPR/R5020 or the human glucocorticoid receptor/dexamethasone (hGR/Dex) complex further supported that hPR/RU486 bound in vivo to its cognate responsive element. Indeed, the observed inhibition of transcription was shown to be due to competition for the MMTV PRE, since no transcriptional interference by the hPR/RU486 was observed, and since no heterodimers were formed between hPR/RU486 and cPR/R5020 or hGR/Dex. That the ligand-free hPR, however, was unable to compete, demonstrated that ligand binding is the prerequisite for DNA binding of hPR in vivo. Previous ArticleNext Article Volume 9Issue 121 December 1990In this issue RelatedDetailsLoading ..." @default.
• W74071586 created "2016-06-24" @default.
• W74071586 creator A5019481782 @default.
• W74071586 creator A5037309838 @default.
• W74071586 creator A5044999679 @default.
• W74071586 creator A5067169499 @default.
• W74071586 creator A5083990330 @default.
• W74071586 creator A5085761422 @default.
• W74071586 date "1990-12-01" @default.
• W74071586 modified "2023-10-18" @default.
• W74071586 title "Agonistic and antagonistic activities of RU486 on the functions of the human progesterone receptor." @default.
• W74071586 cites W1569704074 @default.
• W74071586 cites W1571431043 @default.
• W74071586 cites W1575847274 @default.
• W74071586 cites W1579135337 @default.
• W74071586 cites W1589784623 @default.
• W74071586 cites W1596341727 @default.
• W74071586 cites W1598811569 @default.
• W74071586 cites W1598886839 @default.
• W74071586 cites W1812050158 @default.
• W74071586 cites W1843244908 @default.
• W74071586 cites W1851222925 @default.
• W74071586 cites W196155416 @default.
• W74071586 cites W1969489572 @default.
• W74071586 cites W1971960644 @default.
• W74071586 cites W1979801990 @default.
• W74071586 cites W1992871476 @default.
• W74071586 cites W1997514802 @default.
• W74071586 cites W2002914909 @default.
• W74071586 cites W2005914718 @default.
• W74071586 cites W2007587099 @default.
• W74071586 cites W2008822380 @default.
• W74071586 cites W2010234214 @default.
• W74071586 cites W2010499768 @default.
• W74071586 cites W2014107736 @default.
• W74071586 cites W2025267983 @default.
• W74071586 cites W2029475329 @default.
• W74071586 cites W2033777731 @default.
• W74071586 cites W2050579727 @default.
• W74071586 cites W2060180221 @default.
• W74071586 cites W2060484288 @default.
• W74071586 cites W2060903877 @default.
• W74071586 cites W2066441904 @default.
• W74071586 cites W2071525868 @default.
• W74071586 cites W2073836271 @default.
• W74071586 cites W2077679480 @default.
• W74071586 cites W2085124463 @default.
• W74071586 cites W2088591863 @default.
• W74071586 cites W2107620873 @default.
• W74071586 cites W2123015098 @default.
• W74071586 cites W2159545772 @default.
• W74071586 cites W224890781 @default.
• W74071586 cites W40918469 @default.
• W74071586 doi "https://doi.org/10.1002/j.1460-2075.1990.tb07613.x" @default.
• W74071586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/552163" @default.
• W74071586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2249658" @default.
• W74071586 hasPublicationYear "1990" @default.
• W74071586 type Work @default.
• W74071586 sameAs 74071586 @default.
• W74071586 citedByCount "316" @default.
• W74071586 countsByYear W740715862012 @default.
• W74071586 countsByYear W740715862013 @default.
• W74071586 countsByYear W740715862014 @default.
• W74071586 countsByYear W740715862015 @default.
• W74071586 countsByYear W740715862016 @default.
• W74071586 countsByYear W740715862018 @default.
• W74071586 countsByYear W740715862019 @default.
• W74071586 countsByYear W740715862020 @default.
• W74071586 countsByYear W740715862021 @default.
• W74071586 countsByYear W740715862022 @default.
• W74071586 countsByYear W740715862023 @default.
• W74071586 crossrefType "journal-article" @default.
• W74071586 hasAuthorship W74071586A5019481782 @default.
• W74071586 hasAuthorship W74071586A5037309838 @default.
• W74071586 hasAuthorship W74071586A5044999679 @default.
• W74071586 hasAuthorship W74071586A5067169499 @default.
• W74071586 hasAuthorship W74071586A5083990330 @default.
• W74071586 hasAuthorship W74071586A5085761422 @default.
• W74071586 hasBestOaLocation W740715861 @default.
• W74071586 hasConcept C104317684 @default.
• W74071586 hasConcept C121608353 @default.
• W74071586 hasConcept C138885662 @default.
• W74071586 hasConcept C150194340 @default.
• W74071586 hasConcept C153911025 @default.
• W74071586 hasConcept C161733203 @default.
• W74071586 hasConcept C170493617 @default.
• W74071586 hasConcept C179926584 @default.
• W74071586 hasConcept C202751555 @default.
• W74071586 hasConcept C2777366897 @default.
• W74071586 hasConcept C2778938600 @default.
• W74071586 hasConcept C41895202 @default.
• W74071586 hasConcept C530470458 @default.
• W74071586 hasConcept C53345823 @default.
• W74071586 hasConcept C54355233 @default.
• W74071586 hasConcept C55493867 @default.
• W74071586 hasConcept C84606932 @default.
• W74071586 hasConcept C86803240 @default.
• W74071586 hasConcept C95444343 @default.

• next