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• W74384520 abstract "The rates of DNA elongation by wild-type phage T4 and a gene 52 DNA-delay am mutant were estimated by pulse labeling infected cells with /_ ^K/thymidine and visualizing the gently extracted DNA by auto­ radiography. The estimated rate of chain elongation of wild-type DNA was 7^9 nucleotides/second (n/sec) early in synthesis and 516 to 5^1 n/sec at a later time. The rate of DNA elongation by the am mutant was measured to be 693 n/sec, 758 n/sec and 829 n/sec during successive stages of synthesis indicating that elongation was not slower than in wild-type. The kinetics of DNA increase after infection of host cells by wild-type phage T^ or by the gene 52 DNA-delay am mutant was followed using j/ ^H/thymidine uptake into acid insoluble material. From the rates of DNA increase and the rates of DNA elongation we were able to estimate the number of growing points per chromosome equivalent of tem­ plate DNA during the exponential and linear phases. Our estimates for wild-type phage were 0.55 and 0.71-0.80 growing points per chromosome equivalent of template DNA in the exponential and linear phases re­ spectively. For the am mutant we found O.l1* and 0.12-0.13 growing points per chromosome equivalent of template DNA during the exponential and linear phases respectively. The apparent lower incidence of grow­ ing points in the am mutant infections suggests that the mutant may be defective in the initiation of growing points." @default.
• W74384520 created "2016-06-24" @default.
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• W74384520 date "1978-01-01" @default.
• W74384520 modified "2023-09-27" @default.
• W74384520 title "ROLE OF E. COLI GYRASE AND PHAGE T4 DNA-DELAY GENE PRODUCTS IN INITIATION OF PHAGE DNA REPLICATION" @default.
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• W74384520 hasPublicationYear "1978" @default.
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