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• W74425936 abstract "Chlamydomonas reinhardtii is a popular model organism in modern cell biology. Historically, methods for preparing this cell for transmission electron microscopy have used conventional chemical fixation that can result in artifacts that affect the 3-D organization of the cell. We have developed improved methods of specimen preparation that involve high-pressure freezing followed by freeze-substitution that are particularly well suited for 3-D studies (O'Toole et al., 2003, 2007). In this chapter, we describe the details of our cryopreparation methods for the optimal preservation of whole cells for immunocytochemistry and electron tomography. Examples are presented that show the utility of this approach for studying the 3-D architecture of membrane systems and cytoskeletal arrays in intact cells." @default.
• W74425936 created "2016-06-24" @default.
• W74425936 creator A5030709873 @default.
• W74425936 date "2010-01-01" @default.
• W74425936 modified "2023-09-26" @default.
• W74425936 title "Chlamydomonas" @default.
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• W74425936 doi "https://doi.org/10.1016/s0091-679x(10)96004-4" @default.
• W74425936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20869519" @default.
• W74425936 hasPublicationYear "2010" @default.
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