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• W747161775 abstract "Here, we report the development of an electric field-assisted methodology for constructing 3D C2C12 cell sheets with the potential for cell surface modification. In this method, a conducting polymer, polypyrrole (Ppy), is electrodeposited via biotin doping, and then chemical conjugation of biotinylated bone morphogenetic protein 2 (BMP2) is achieved using a biotin-streptavidin cross-linker. Subsequently, C2C12 cells are cultured on BMP2-immobilized Ppy surfaces to induce interactions between cell surface receptors and bound BMP2 ligands. Following these procedures, layers of BMP2-immobilized cells can be easily detached from the Ppy surface by applying an electrical potential. This novel method results in high affinity, ligand-bound cell sheets, which exhibit homogeneous coverage with membrane-bound proteins and signal activation that occurs via maximal receptor accessibility. Using this strategy to engineer the cell surface with desirable ligands results in structures that mimic in vivo tissues; thus, the method reported here has potential applications in regenerative medicine and tissue engineering." @default.
• W747161775 created "2016-06-24" @default.
• W747161775 creator A5003841335 @default.
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• W747161775 date "2015-01-01" @default.
• W747161775 modified "2023-10-14" @default.
• W747161775 title "An Innovative Strategy for the Fabrication of Functional Cell Sheets Using an Electroactive Conducting Polymer" @default.
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• W747161775 doi "https://doi.org/10.7150/thno.12389" @default.
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