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• W748185973 abstract "Hypericin is an agent extracted from Hypericum plants. St. John s wort (Hypericum perforatum, L.) is the most common representative of this genus. Blooms and leaves of Hypericum plants are covered with hypericin-containing glands. Hypericin probably protects the plants from herbivorous insects. This may relate to the photosensitizing properties of this agent. Once diffused into cells, hypericin causes massive cell damage under light exposition due to oxidative stress based on the generation of singlet oxygen. Hypericin seems to be a multikinase inhibitor as well. In this study the effects of hypericin on human renal carcinoma cells were investigated for the first time. Hypericin accumulates in the endoplasmatic reticulum and in the mitochondria but not in the nucleus of renal carcinoma cells. After intracellular uptake hypericin shows an intensive and stable red fluorescence, that is even detectable after 48 hours. Under dark conditions hypericin affect neither metabolic activity nor colony forming ability of the cells. The treatment of renal carcinoma cells with hypericin in combination with illumination leads to a significant decline of metabolic activity and colony forming ability. Futhermore apoptosis is induced in 100% of the cells through this treatment. These phototoxic effects were caused through intracellular formation of reactive oxygen species mainly singlett oxygen that is proved through redoxsensitive fluorescence dye (roGFP). First in this work a significant radiosensitizing effect of hypericin on the very radioresistent renal carcinoma cells could be shown as well. The colony forming ability of renal carcinoma cells after irradiation is significant reduced through hypericin. The metabolic activity of the cells increases after irradiation independent of hypericin treatment. An irradiation with a dose of 2Gy or 4Gy induces apoptosis in only 2,5% or 5% of the cells independent from hypericin treatment. This results suggest that an irradiation in combination with hypericin induces mitotic cell death o f renal carcinoma cells. The radiosensitizing effects of hypericin probably base on inhibition of DNA-repair mechanisms. The results of this work give first advice that the expression and activity of different proteins that are involved in DNA-repair mechanisms, is reduced or slow down through hypericin. That probably leads to a decreased clonogenic survival of the cells after irradiation and that results in an enhanced radiosensitivity of renal carcinoma cells." @default.
• W748185973 created "2016-06-24" @default.
• W748185973 creator A5065263766 @default.
• W748185973 date "2022-02-18" @default.
• W748185973 modified "2023-09-27" @default.
• W748185973 title "Effekte von Hypericin auf humane renale Karzinomzellen in vitro" @default.
• W748185973 doi "https://doi.org/10.53846/goediss-361" @default.
• W748185973 hasPublicationYear "2022" @default.
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