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- W748678030 abstract "Castor-oil, extracted from seeds of Ricinus communis L., is normally applied in natura or in its modified form in medical, pharmaceutical and industrial areas. One of its chemical derivates – the Poliquilgerm showed antifungal properties on Candida albicans, and bacteriostatic/bactericide ones on Escherichia coli reaching 99.9%. Considering these features, Poliquilgerm was applied in cultures of Leuconostoc mesenteroides, a frequent contaminant bacterium of musts on sugar and alcohol industries. This bacterium when contaminant of must produce beyond lactic acid the dextran, a polymer of glucose, with gelatinous consistency which may increase the operation fluids viscosity. Due to these characteristics, in the present study, the effect of different concentrations of Poliquilgerm on viscosity of dextran’s solutions and on cultures of L. mesenteroides was evaluated. Analyzing the solutions of dextrana in contact with the product, it was concluded that it did not induce hydrolysis on glycosidic links. The product in concentrations of 1.0 and 0.2% inhibited the growth of L. mesenteroides reaching up to 100% in both concentrations, as evidenced by biomass quantification and confirmed by plating by Pour Plate technique, which inhibition was 98% of decrease in the CFU / mL after 24 hours in contact with 1.0% of the product. In relation to the culture viscosity, it was observed 20.56% of reduction when it was used 1.0% of the product. In mixed culture (L. mesenteroides and S. cerevisiae), it was registered up to 6.8% of decrease in viscosity. It was observed that S. Cerevisiae shows sensitivity to 1.0 and 0.2% concentrations of Poliquilgerm only at the initial time, because after 24 hours the culture reaches the same level of growth control, getting adaptated to the presence of the product. It was studied the Poliquilgerm hydrolytic activity on release of ART from live and dead cells of L. fermentum, E. coli, S. cerevisiae and C. albicans, submitted to centrifugation and washing of cells. The results were insignificant to ART released. This, allied with the no hydrolysis of dextrana, can suppose that the action of Poliquilgerm could be interfering in another cell site to induce the death of cells. Key-words: Poliquilgerm , dextran, Leuconostoc mesenteroides" @default.
- W748678030 created "2016-06-24" @default.
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- W748678030 date "2008-06-12" @default.
- W748678030 modified "2023-09-24" @default.
- W748678030 title "Estudo do composto derivado do óleo de Ricinus communis L. (mamona) sobre a bactéria e biopolímero da fermantação etanólica" @default.
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