FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W75015331> ?p ?o ?g. }

• W75015331 endingPage "6" @default.
• W75015331 startingPage "231" @default.
• W75015331 abstract "This study was carried out to determine the role of non-biting synanthropic flies as carriers of Cryptosporidium spp. oocysts in the vicinity of the city of Gdańsk (NE Poland). In 2001-2003, flies were collected from three breeding sites: cow sheds and meadows in the Bystra cattle farm and municipal landfill Szadółki using inhaustors (aspirators) and entomologic nets. A total of 2358 specimens of the families: Muscidae (n = 1598), Calliphoridae (n = 739) and Sarcophagidae (n =21) were collected and analysed in 249 pools consisted of 9.5 insects, in average. Microscopic examination was used to detect Cryptosporidium spp. oocysts in the fly faeces deposited on the glass microscope slides and stained by Zhiel-Nielsen method. The mean number of faecal droplets per one glass slide was 11.5. Ooocysts of Cryptosporidium spp., stained from light pink to bright red, were found in fly faeces deposited on 25 (27.5%) of 91 glass slides checked. The highest prevalence of the pathogen was observed in faecal droplets deposited by flies collected in municipal landfill (50% investigated slides). DNA of Cryptosporidium spp. was extracted from the surface eluants of flies and/or their gut homogenates and purified. Then extracts were examined by PCR using CPB-DIAGF and CPB-DIAGR primers amplifying a variable region SSU-rRNA of all Cryptosporidium species. Altogether 387 isolates, 228 from surfaces and 159 from gut homogenates, were obtained from 249 pools of flies and analyzed. A specific 435 bp fragment of DNA was obtained in 49 (12.7%) lysates tested. In 10.4% pools, DNA of the pathogen was detected only in the surface eluants while in 7.6% only in gut extracts. In the case of two pooled samples (0.8%) Cryptosporidium spp. was found in both types of lysates. In total, Cryptosporidium spp. was detected in 47/249 pools of flies (18.9%). Assumed that each positive pool contained just one infected fly, the percentage of specimens able to oocysts transmission were calculated at the minimal level 2.0% (n = 47/2358). The result confirm that synanthropic flies can harbour oocysts of Cryptosporidium spp. both externally and internally, and disseminate them mechanically in the environment. Therefore, under unsanitary conditions they could be involved in the transmission of human and animal cryptosporidiosis." @default.
• W75015331 created "2016-06-24" @default.
• W75015331 creator A5046541605 @default.
• W75015331 creator A5076356613 @default.
• W75015331 creator A5086742654 @default.
• W75015331 creator A5088701364 @default.
• W75015331 date "2009-01-01" @default.
• W75015331 modified "2023-10-16" @default.
• W75015331 title "[The occurrence of Cryptosporidium spp. in synanthropic flies in urban and rural environments]." @default.
• W75015331 cites W1998241167 @default.
• W75015331 cites W2035079649 @default.
• W75015331 cites W2036874382 @default.
• W75015331 cites W2074904358 @default.
• W75015331 cites W2087862123 @default.
• W75015331 cites W2108870664 @default.
• W75015331 cites W2121959409 @default.
• W75015331 cites W2122981229 @default.
• W75015331 cites W2134353100 @default.
• W75015331 cites W2137009284 @default.
• W75015331 cites W2144647173 @default.
• W75015331 cites W2157867349 @default.
• W75015331 cites W2334039896 @default.
• W75015331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19856840" @default.
• W75015331 hasPublicationYear "2009" @default.
• W75015331 type Work @default.
• W75015331 sameAs 75015331 @default.
• W75015331 citedByCount "1" @default.
• W75015331 crossrefType "journal-article" @default.
• W75015331 hasAuthorship W75015331A5046541605 @default.
• W75015331 hasAuthorship W75015331A5076356613 @default.
• W75015331 hasAuthorship W75015331A5086742654 @default.
• W75015331 hasAuthorship W75015331A5088701364 @default.
• W75015331 hasConcept C173758957 @default.
• W75015331 hasConcept C182049041 @default.
• W75015331 hasConcept C18903297 @default.
• W75015331 hasConcept C2776460866 @default.
• W75015331 hasConcept C2778207175 @default.
• W75015331 hasConcept C2778877831 @default.
• W75015331 hasConcept C2780039072 @default.
• W75015331 hasConcept C42972112 @default.
• W75015331 hasConcept C61716771 @default.
• W75015331 hasConcept C71924100 @default.
• W75015331 hasConcept C86803240 @default.
• W75015331 hasConcept C89423630 @default.
• W75015331 hasConcept C90856448 @default.
• W75015331 hasConceptScore W75015331C173758957 @default.
• W75015331 hasConceptScore W75015331C182049041 @default.
• W75015331 hasConceptScore W75015331C18903297 @default.
• W75015331 hasConceptScore W75015331C2776460866 @default.
• W75015331 hasConceptScore W75015331C2778207175 @default.
• W75015331 hasConceptScore W75015331C2778877831 @default.
• W75015331 hasConceptScore W75015331C2780039072 @default.
• W75015331 hasConceptScore W75015331C42972112 @default.
• W75015331 hasConceptScore W75015331C61716771 @default.
• W75015331 hasConceptScore W75015331C71924100 @default.
• W75015331 hasConceptScore W75015331C86803240 @default.
• W75015331 hasConceptScore W75015331C89423630 @default.
• W75015331 hasConceptScore W75015331C90856448 @default.
• W75015331 hasIssue "3" @default.
• W75015331 hasLocation W750153311 @default.
• W75015331 hasOpenAccess W75015331 @default.
• W75015331 hasPrimaryLocation W750153311 @default.
• W75015331 hasRelatedWork W192134533 @default.
• W75015331 hasRelatedWork W2134353100 @default.
• W75015331 hasRelatedWork W2186809532 @default.
• W75015331 hasRelatedWork W2327708007 @default.
• W75015331 hasRelatedWork W2340637240 @default.
• W75015331 hasRelatedWork W2418158274 @default.
• W75015331 hasRelatedWork W2524790499 @default.
• W75015331 hasRelatedWork W3146343873 @default.
• W75015331 hasRelatedWork W75015331 @default.
• W75015331 hasRelatedWork W2345991545 @default.
• W75015331 hasVolume "55" @default.
• W75015331 isParatext "false" @default.
• W75015331 isRetracted "false" @default.
• W75015331 magId "75015331" @default.
• W75015331 workType "article" @default.