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- W750196813 abstract "ABSTRACT Both normal rat pancreatic islet β-cells and insulin-producing BRIN-BD11 cells were recently reported to be equipped with the Na + -HCO 3- -cotransporter NBC1 [1,2]. In the present study, four distinct experimental designs were used to assess the possible effects of an inhibitor of NBC1, i.e. tenidap (0.1 mM), upon the efflux of Na + from insulin-producing cells. First, isolated rat pancreatic islets were prelabelled with 22 Na + and, after washing, incubated for 10 min in a non- radioactive medium, the final radioactivity content of the islets and of the incubation medium being eventually measured. A comparable procedure was then applied to dispersed rat pancreatic islet cells. In the third approach, dispersed rat pancreatic islet cells were incubated for 5, 10 and 20 min in the presence of 22 Na + , and then separate from the incubation medium and examined for their radioactive content. Last, a comparable approach was applied to insulin– producing BRIN-BD11 cells. The latter approach allowed to document that tenidap indeed increases the net uptake of" @default.
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- W750196813 date "2009-01-01" @default.
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- W750196813 title "Effect of tenidap on sodium fluxes in pancreatic islet cells: methodological aspects" @default.
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