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- W75146820 endingPage "387" @default.
- W75146820 startingPage "374" @default.
- W75146820 abstract "This chapter describes the activity and the specificity of thermolysin and related bacillus metallopeptidases. The pH-activity profiles of thermolysin and Thermolysin-Like Proteases (TLPs) follow a bell-shaped curve with maximal activity at or near pH 7.0 for both proteolysis. Mature thermolysin comprises 316 residues, forming a bilobal structure in which the N-terminal region is predominantly β-pleated sheet and the C-terminal domain is predominantly α-helical. The catalytically essential zinc atom is located in a deep active-site cleft that lies between the two lobes. The crystallographic studies on thermolysin, coupled with parallel studies on the zinc endopeptidase carboxypeptidase A, have led to a general base mechanism of action being proposed for the zinc peptidase family, with the glutamate of the HEALTH sequence polarizing the zinc-bound water molecule which subsequently attacks the scissile bond. Another histidine residue is proposed to contribute by stabilizing the transition state. Comparison of the crystal structures of thermolysin–ligand complexes with the structure of the free enzyme strongly indicates that thermolysin undergoes a conformational change upon substrate or inhibitor binding." @default.
- W75146820 created "2016-06-24" @default.
- W75146820 creator A5021809226 @default.
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- W75146820 creator A5044807184 @default.
- W75146820 creator A5052089482 @default.
- W75146820 creator A5087281474 @default.
- W75146820 date "2004-01-01" @default.
- W75146820 modified "2023-09-26" @default.
- W75146820 title "Thermolysin and related Bacillus metallopeptidases" @default.
- W75146820 cites W1487189791 @default.
- W75146820 cites W1491832097 @default.
- W75146820 cites W1507141206 @default.
- W75146820 cites W1510807601 @default.
- W75146820 cites W1513500933 @default.
- W75146820 cites W1530747269 @default.
- W75146820 cites W1532241593 @default.
- W75146820 cites W1540442759 @default.
- W75146820 cites W1544020507 @default.
- W75146820 cites W1572975248 @default.
- W75146820 cites W1581901221 @default.
- W75146820 cites W1604702970 @default.
- W75146820 cites W1649274438 @default.
- W75146820 cites W1703401278 @default.
- W75146820 cites W1779662607 @default.
- W75146820 cites W1806837705 @default.
- W75146820 cites W1811590717 @default.
- W75146820 cites W1841321786 @default.
- W75146820 cites W1908837363 @default.
- W75146820 cites W1930865184 @default.
- W75146820 cites W1939390578 @default.
- W75146820 cites W1968857660 @default.
- W75146820 cites W1975132550 @default.
- W75146820 cites W1975198669 @default.
- W75146820 cites W1975691906 @default.
- W75146820 cites W1976755595 @default.
- W75146820 cites W1977794986 @default.
- W75146820 cites W1977878533 @default.
- W75146820 cites W1979094001 @default.
- W75146820 cites W1980694228 @default.
- W75146820 cites W1981119403 @default.
- W75146820 cites W1983435932 @default.
- W75146820 cites W1987645466 @default.
- W75146820 cites W1989743694 @default.
- W75146820 cites W1989850678 @default.
- W75146820 cites W1990259481 @default.
- W75146820 cites W1990720548 @default.
- W75146820 cites W1994048807 @default.
- W75146820 cites W1994083018 @default.
- W75146820 cites W1994570307 @default.
- W75146820 cites W1997388207 @default.
- W75146820 cites W1997541998 @default.
- W75146820 cites W2000794695 @default.
- W75146820 cites W2002970490 @default.
- W75146820 cites W2003424499 @default.
- W75146820 cites W2006511206 @default.
- W75146820 cites W2006645661 @default.
- W75146820 cites W2007142690 @default.
- W75146820 cites W2013670421 @default.
- W75146820 cites W2016131951 @default.
- W75146820 cites W2016784305 @default.
- W75146820 cites W2017176515 @default.
- W75146820 cites W2017565306 @default.
- W75146820 cites W2019285471 @default.
- W75146820 cites W2019962781 @default.
- W75146820 cites W2021275615 @default.
- W75146820 cites W2021531856 @default.
- W75146820 cites W2021577368 @default.
- W75146820 cites W2023731100 @default.
- W75146820 cites W2026547695 @default.
- W75146820 cites W2027027784 @default.
- W75146820 cites W2027907587 @default.
- W75146820 cites W2028300103 @default.
- W75146820 cites W2029030041 @default.
- W75146820 cites W2029388371 @default.
- W75146820 cites W2029686855 @default.
- W75146820 cites W2030593791 @default.
- W75146820 cites W2031787227 @default.
- W75146820 cites W2032751771 @default.
- W75146820 cites W2033880987 @default.
- W75146820 cites W2034934568 @default.
- W75146820 cites W2037256823 @default.
- W75146820 cites W2039335288 @default.
- W75146820 cites W2039634842 @default.
- W75146820 cites W2039771104 @default.
- W75146820 cites W2039922821 @default.
- W75146820 cites W2041532829 @default.
- W75146820 cites W2041837484 @default.
- W75146820 cites W2041977189 @default.
- W75146820 cites W2043110757 @default.
- W75146820 cites W2043686473 @default.
- W75146820 cites W2045376606 @default.
- W75146820 cites W2046601398 @default.
- W75146820 cites W2047306549 @default.
- W75146820 cites W2047555806 @default.
- W75146820 cites W2049115197 @default.
- W75146820 cites W2049521571 @default.
- W75146820 cites W2050868165 @default.