Matches in SemOpenAlex for { <https://semopenalex.org/work/W75167679> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W75167679 abstract "A four-hit chromosome-specific YAC library is being used to construct an overlapping YAC clone physical map of human chromosome 11. Each of the 1824 YAC clones have been sized by pulsed-field gel analysis with a resulting average insert size of 350 kb. Three hundred of the clones have been localized to a chromosome band by fluorescence in situ hybridization (FISH). We are using two complementary approaches to assemble YAC contigs: hybridization-based screening with Alu-PCR products and STS-based screening. Both screening approaches use pooling strategies to uniquely address each of the well locations with a minimum number of DNA pools. Since our main source of YAC clones is a chromosome specific library, the pool complexity is an order of magnitude smaller in comparison to total genomic libraries. Alu products with three different Alu primers were generated from both the monochromosomal J1 hybrid and individual YAC clones. YAC clones were chosen to generate probes using a sample without replacement strategy. Greater than 1700 YAC clones have been assembled into contigs. These initial contigs are estimated to be on average 1 Mb in size. Each of the contigs have been binned into a chromosomal band by virture of it containing YACs localized bymore » FISH or YACs positive for one or more mapped STSs. Confirmation of the contigs is being achieved with Line I fingerprinting. The initial contigs are currently being joined using probes derived from the YAC clone ends defining the outermost points in the contigs for hybridization of colony filters. Remaining gaps in the contigs will be joined using alternative large insert libraries.« less" @default.
- W75167679 created "2016-06-24" @default.
- W75167679 creator A5011783428 @default.
- W75167679 creator A5048567447 @default.
- W75167679 creator A5056352064 @default.
- W75167679 date "1994-09-01" @default.
- W75167679 modified "2023-09-24" @default.
- W75167679 title "Generating a physical map of chromosome 11" @default.
- W75167679 hasPublicationYear "1994" @default.
- W75167679 type Work @default.
- W75167679 sameAs 75167679 @default.
- W75167679 citedByCount "1" @default.
- W75167679 crossrefType "journal-article" @default.
- W75167679 hasAuthorship W75167679A5011783428 @default.
- W75167679 hasAuthorship W75167679A5048567447 @default.
- W75167679 hasAuthorship W75167679A5056352064 @default.
- W75167679 hasConcept C104317684 @default.
- W75167679 hasConcept C122735190 @default.
- W75167679 hasConcept C127413603 @default.
- W75167679 hasConcept C13575015 @default.
- W75167679 hasConcept C141231307 @default.
- W75167679 hasConcept C2777542201 @default.
- W75167679 hasConcept C2780098074 @default.
- W75167679 hasConcept C3017666073 @default.
- W75167679 hasConcept C30481170 @default.
- W75167679 hasConcept C35101798 @default.
- W75167679 hasConcept C54355233 @default.
- W75167679 hasConcept C59582021 @default.
- W75167679 hasConcept C6970260 @default.
- W75167679 hasConcept C70721500 @default.
- W75167679 hasConcept C78519656 @default.
- W75167679 hasConcept C81089528 @default.
- W75167679 hasConcept C86803240 @default.
- W75167679 hasConceptScore W75167679C104317684 @default.
- W75167679 hasConceptScore W75167679C122735190 @default.
- W75167679 hasConceptScore W75167679C127413603 @default.
- W75167679 hasConceptScore W75167679C13575015 @default.
- W75167679 hasConceptScore W75167679C141231307 @default.
- W75167679 hasConceptScore W75167679C2777542201 @default.
- W75167679 hasConceptScore W75167679C2780098074 @default.
- W75167679 hasConceptScore W75167679C3017666073 @default.
- W75167679 hasConceptScore W75167679C30481170 @default.
- W75167679 hasConceptScore W75167679C35101798 @default.
- W75167679 hasConceptScore W75167679C54355233 @default.
- W75167679 hasConceptScore W75167679C59582021 @default.
- W75167679 hasConceptScore W75167679C6970260 @default.
- W75167679 hasConceptScore W75167679C70721500 @default.
- W75167679 hasConceptScore W75167679C78519656 @default.
- W75167679 hasConceptScore W75167679C81089528 @default.
- W75167679 hasConceptScore W75167679C86803240 @default.
- W75167679 hasLocation W751676791 @default.
- W75167679 hasOpenAccess W75167679 @default.
- W75167679 hasPrimaryLocation W751676791 @default.
- W75167679 hasRelatedWork W1987739997 @default.
- W75167679 hasRelatedWork W1999071440 @default.
- W75167679 hasRelatedWork W2021727821 @default.
- W75167679 hasRelatedWork W2022408984 @default.
- W75167679 hasRelatedWork W2032162808 @default.
- W75167679 hasRelatedWork W2041112284 @default.
- W75167679 hasRelatedWork W2064176745 @default.
- W75167679 hasRelatedWork W2066069113 @default.
- W75167679 hasRelatedWork W2078718524 @default.
- W75167679 hasRelatedWork W2120597164 @default.
- W75167679 hasRelatedWork W2123013414 @default.
- W75167679 hasRelatedWork W2125979046 @default.
- W75167679 hasRelatedWork W2130435569 @default.
- W75167679 hasRelatedWork W2133749554 @default.
- W75167679 hasRelatedWork W2134769659 @default.
- W75167679 hasRelatedWork W2157686306 @default.
- W75167679 hasRelatedWork W2382453252 @default.
- W75167679 hasRelatedWork W2405478645 @default.
- W75167679 hasRelatedWork W2407165562 @default.
- W75167679 hasRelatedWork W3192377763 @default.
- W75167679 hasVolume "55" @default.
- W75167679 isParatext "false" @default.
- W75167679 isRetracted "false" @default.
- W75167679 magId "75167679" @default.
- W75167679 workType "article" @default.