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- W752151489 abstract "Dopamine and serotonin are biogenic neurotransmitters that play major roles in the nervous system. In invertebrates, dopamine has been shown to be involved in aversive learning, and serotonin in the processes of olfaction, with the effects of these neurotransmitters being mediated by specific GPCRs. In order to characterize the effects of serotonin in olfaction, pharmacological drugs that regulate both serotonin and dopamine receptors are often used. To characterize the possibility of drug crosstalk between serotonin and dopamine receptors in Manduca sexta, the four suspected dopamine receptors will be characterized to help more accurately study the effects of serotonin on the olfactory system. PCR on whole brain cDNA with degenerate oligonucleotide primers produced an expected 255 bp DNA fragment that corresponds to the Manduca sexta homolog of an INDR-type dopamine receptor found in Bombyx mori and Papilio xuthus. Therefore, a portion of the first dopamine receptor has been successfully cloned. An amino acid change in the cloned sequence suggests that the Manduca sexta homolog may potentially be evolutionarily closer to Papilio xuthus than Bombyx mori, although the full-length clone must be analyzed to determine the phylogenetic significance of this sequence difference." @default.
- W752151489 created "2016-06-24" @default.
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- W752151489 date "2008-09-01" @default.
- W752151489 modified "2023-09-26" @default.
- W752151489 title "THE CLONING OF AN INDR-TYPE DOPAMINE RECEPTOR IN MANDUCA SEXTA" @default.
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