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- W752283992 abstract "Some biochemical effects of β-mercaptoethanol on the liver of mouse and rat The effects of β-mercaptoethanol (HSCH2-CH2OH) on the biochemistry of mouse and rat liver have been studied both in vivo and in vitro. It has been found that intraperitoneal injection of mercaptoethanol has no appreciable effect on the soluble-SH, ATP, DNA and RNA content of the cells. On the other hand, mercaptoethanol exerts a very definite inhibitory effect on the incorporation of labelled amino acids into liver proteins both in vivo and in vitro (30–40% inhibition). Mercaptoethanol, if injected in vivo, does not set free one of the enzymes characteristic of lysosomes, acid phosphatase; there is no liberation either of acid phosphatase when a particulate fraction containing the lysosomes is treated by mercaptoethanol in vitro. The meaning of these results is discussed. Nous avons étudié les effets in vivo et in vitro du β-mercaptoéthanol (HSCH2-CH2OH) sur les propriétés biochimiques du foie de souris et de rat. L'injection intrapéritonéale de mercaptoéthanol n'exerce pas d'effets importants sur la teneur en -SH solubles, ATP, ADN et ARN des cellules. Par contre, le mercaptoéthanol exerce un net effet inhibiteur (30–40% d'inhibition) sur l'incorporation d'acides aminés marqués dans les protéines du foie, tant in vivo qu'in vitro. Enfin, le mercaptoéthanol, lorsqu'on l'injecte in vivo, ne libère pas l'un des enzymes caractéristiques des lysosomes, la phosphatase acide; il n'y a pas non plus de libération de cet enzyme lorsqu'on traite in vitro par le mercaptoéthanol la fraction de l'homogénat la plus riche en lysosomes. La signification de ces divers résultats est discutée." @default.
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- W752283992 date "1961-08-01" @default.
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- W752283992 title "Quelques effets biochimiques du β-mercaptoéthanol sur le foie de souris et de rat" @default.
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- W752283992 doi "https://doi.org/10.1016/0006-3002(61)90176-7" @default.
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