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- W754945189 abstract "Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic an - tioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were col - lected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10 -3 U · l -1 , 2.65·10 -3 U · l -1 , and 525 U · l -1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·10 3 mol · l -1 , 33.3·10 3 mol · l -1 , and 133·10 -9 mol · l -1 , re - spectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher com - pared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences." @default.
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- W754945189 date "2011-01-01" @default.
- W754945189 modified "2023-09-26" @default.
- W754945189 title "ACTIVITY OF ANTIOXIDANT ENZYMES AND CONCENTRATION OF MN, CU, ZN, AND PROTEIN IN PORCINE FOLLICULAR FLUID" @default.
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