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• W755221254 abstract "In matrix-assisted ionization (MAI), analyte incorporated in a small molecule matrix is introduced into an aperture linking atmospheric pressure with the vacuum of a mass spectrometer. Gas-phase analyte ions are spontaneously produced without use of a laser or high voltage. Here we investigate analyte and background ion abundances upon addition of ammonium salts to various MAI matrix/analyte solutions. Regardless of the ammonium salt or matrix used, chemical background ions are suppressed and/or analyte ion abundance improved for basic small molecules, peptides, and proteins. Background ion abundances increase with increasing inlet temperature, but are suppressed with addition of any of a variety of ammonium salts without much effect on the total ion abundances of the analyte ions. However, at lower inlet temperature using the matrices 2-bromo-2-nitropropane-1,3-diol, 1,2-dicyanobenzene, and 3-nitrobenzonitrile (3-NBN), analyte ion abundance increases and any chemical background decreases upon addition of ammonium salt. The improvement in sensitivity using 3-NBN with ammonium salt allows full acquisition mass spectra consuming as little as 1 fmol of ubiquitin. More complete peptide coverage for 100 fmol of a BSA tryptic digest, and increased sensitivity of drugs spiked in urine and saliva were observed after ammonium salt addition to the 3-NBN matrix." @default.
• W755221254 created "2016-06-24" @default.
• W755221254 creator A5018214593 @default.
• W755221254 creator A5073804963 @default.
• W755221254 date "2015-06-30" @default.
• W755221254 modified "2023-10-13" @default.
• W755221254 title "Improving the Sensitivity of Matrix-Assisted Ionization (MAI) Mass Spectrometry Using Ammonium Salts" @default.
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• W755221254 doi "https://doi.org/10.1007/s13361-015-1205-z" @default.
• W755221254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26122522" @default.
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