Matches in SemOpenAlex for { <https://semopenalex.org/work/W75575892> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W75575892 endingPage "C1086" @default.
- W75575892 startingPage "C1077" @default.
- W75575892 abstract "Cystic fibrosis (CF) airway epithelia exhibit enhanced Na+ reabsorption in parallel with diminished Cl- secretion. We tested the hypothesis that actin plays a role in the regulation of a cloned epithelial Na+ channel (ENaC) by the cystic fibrosis transmembrane conductance regulator (CFTR). We found that immunopurified bovine tracheal CFTR coreconstituted into a planar lipid bilayer with alpha,beta,gamma-rat ENaC (rENaC) decreased single-channel open probability (Po) of rENaC in the presence of actin by over 60%, a significantly greater effect than was observed in the absence of actin (approximately 20%). In the presence of actin, protein kinase A plus ATP activated both CFTR and rENaC, but CFTR was activated in a sustained manner, whereas the activation of rENaC was transitory. ATP alone could also activate ENaC transiently in the presence ofactin but had no effect on CFTR. Stabilizing short actin filaments at a fixed length with gelsolin (at a ratio to actin of 2:1) produced a sustained activation of alpha,beta,gamma-rENaC in both the presence or absence of CFTR. Gelsolin alone (i.e., in the absence of actin) had no effect on the conductance or Po of either CFTR or rENaC. We have also found that short actin filaments produced their modulatory action on alpha-rENaC independent of the presence of the beta- or gamma-rENaC subunits. In contrast, CFTR did not affect any properties of the channel formed by alpha-rENaC alone, i.e., in the absence of beta- or gamma-rENaC. These results indicate that CFTR can directly downregulate single Na+ channel activity, which may account for the observed differences between Na+ transport in normal and CF-affected airway epithelia. Moreover, the presence of actin confers an enhanced modulatory ability of CFTR on Na+ channels." @default.
- W75575892 created "2016-06-24" @default.
- W75575892 creator A5008621245 @default.
- W75575892 creator A5023640581 @default.
- W75575892 creator A5030792958 @default.
- W75575892 creator A5036056918 @default.
- W75575892 creator A5040712845 @default.
- W75575892 creator A5042453107 @default.
- W75575892 creator A5048753485 @default.
- W75575892 creator A5064349863 @default.
- W75575892 creator A5065381860 @default.
- W75575892 date "1997-04-01" @default.
- W75575892 modified "2023-10-16" @default.
- W75575892 title "Role of actin in regulation of epithelial sodium channels by CFTR" @default.
- W75575892 cites W1510835878 @default.
- W75575892 cites W1558046708 @default.
- W75575892 cites W1969996729 @default.
- W75575892 cites W1977987849 @default.
- W75575892 cites W1988101098 @default.
- W75575892 cites W2012329129 @default.
- W75575892 cites W2028171116 @default.
- W75575892 cites W2062364311 @default.
- W75575892 cites W2078282757 @default.
- W75575892 cites W2087499467 @default.
- W75575892 cites W2090117730 @default.
- W75575892 cites W2095852063 @default.
- W75575892 cites W2126458759 @default.
- W75575892 cites W2133136550 @default.
- W75575892 cites W2144248619 @default.
- W75575892 cites W2145970171 @default.
- W75575892 cites W2161220285 @default.
- W75575892 cites W2425636262 @default.
- W75575892 doi "https://doi.org/10.1152/ajpcell.1997.272.4.c1077" @default.
- W75575892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9142832" @default.
- W75575892 hasPublicationYear "1997" @default.
- W75575892 type Work @default.
- W75575892 sameAs 75575892 @default.
- W75575892 citedByCount "97" @default.
- W75575892 countsByYear W755758922012 @default.
- W75575892 countsByYear W755758922013 @default.
- W75575892 countsByYear W755758922014 @default.
- W75575892 countsByYear W755758922017 @default.
- W75575892 countsByYear W755758922020 @default.
- W75575892 countsByYear W755758922021 @default.
- W75575892 crossrefType "journal-article" @default.
- W75575892 hasAuthorship W75575892A5008621245 @default.
- W75575892 hasAuthorship W75575892A5023640581 @default.
- W75575892 hasAuthorship W75575892A5030792958 @default.
- W75575892 hasAuthorship W75575892A5036056918 @default.
- W75575892 hasAuthorship W75575892A5040712845 @default.
- W75575892 hasAuthorship W75575892A5042453107 @default.
- W75575892 hasAuthorship W75575892A5048753485 @default.
- W75575892 hasAuthorship W75575892A5064349863 @default.
- W75575892 hasAuthorship W75575892A5065381860 @default.
- W75575892 hasConcept C125705527 @default.
- W75575892 hasConcept C126322002 @default.
- W75575892 hasConcept C178790620 @default.
- W75575892 hasConcept C185592680 @default.
- W75575892 hasConcept C2776938444 @default.
- W75575892 hasConcept C2778428886 @default.
- W75575892 hasConcept C537181965 @default.
- W75575892 hasConcept C6507245 @default.
- W75575892 hasConcept C71924100 @default.
- W75575892 hasConcept C80722340 @default.
- W75575892 hasConcept C86803240 @default.
- W75575892 hasConcept C95444343 @default.
- W75575892 hasConceptScore W75575892C125705527 @default.
- W75575892 hasConceptScore W75575892C126322002 @default.
- W75575892 hasConceptScore W75575892C178790620 @default.
- W75575892 hasConceptScore W75575892C185592680 @default.
- W75575892 hasConceptScore W75575892C2776938444 @default.
- W75575892 hasConceptScore W75575892C2778428886 @default.
- W75575892 hasConceptScore W75575892C537181965 @default.
- W75575892 hasConceptScore W75575892C6507245 @default.
- W75575892 hasConceptScore W75575892C71924100 @default.
- W75575892 hasConceptScore W75575892C80722340 @default.
- W75575892 hasConceptScore W75575892C86803240 @default.
- W75575892 hasConceptScore W75575892C95444343 @default.
- W75575892 hasIssue "4" @default.
- W75575892 hasLocation W755758921 @default.
- W75575892 hasLocation W755758922 @default.
- W75575892 hasOpenAccess W75575892 @default.
- W75575892 hasPrimaryLocation W755758921 @default.
- W75575892 hasRelatedWork W110347682 @default.
- W75575892 hasRelatedWork W1566486303 @default.
- W75575892 hasRelatedWork W1990687915 @default.
- W75575892 hasRelatedWork W1991072550 @default.
- W75575892 hasRelatedWork W2039705009 @default.
- W75575892 hasRelatedWork W2073700577 @default.
- W75575892 hasRelatedWork W2110464131 @default.
- W75575892 hasRelatedWork W2132471653 @default.
- W75575892 hasRelatedWork W3164444686 @default.
- W75575892 hasRelatedWork W2182664732 @default.
- W75575892 hasVolume "272" @default.
- W75575892 isParatext "false" @default.
- W75575892 isRetracted "false" @default.
- W75575892 magId "75575892" @default.
- W75575892 workType "article" @default.