Matches in SemOpenAlex for { <https://semopenalex.org/work/W75589748> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W75589748 endingPage "43" @default.
- W75589748 startingPage "438" @default.
- W75589748 abstract "The gene encoding Thermus filiformis (Tfi) DNA ligase was cloned and its nucleotide sequence was determined by the chain-termination method. The primary structure of Tfi DNA ligase was deduced from its nucleotide sequence. The Tfi DNA ligase comprises of 667 amino acid residues and its molecular mass was determined to be 75,936 Da. The deduced amino acid sequence of Tfi DNA ligase showed a 86.5% homology to Tth DNA ligase and 43.5% to E. coli DNA ligase. The Lys-116 of Lys-Val-Asp-Gly motif was proposed to be the active residue of Tfi DNA ligase. In comparison with the amino acid composition of DNA ligase, Tfi DNA ligase showed a significant increase in the proportion of charged residues, Arg and Glu, compared to E. coli DNA ligase. The G + C content in the first, second, and third positions of the codons used were 70.3%, 40.3%, and 90.3%, respectively. Codon usage in Tfi DNA ligase was heavily biased towards the use of G + C in the third position. Under tac promoter control, Tfi DNA ligase was overproduced to greater than 9% of E. coli BL26Blue cellular proteins." @default.
- W75589748 created "2016-06-24" @default.
- W75589748 creator A5051265114 @default.
- W75589748 creator A5084232009 @default.
- W75589748 date "1998-08-31" @default.
- W75589748 modified "2023-09-23" @default.
- W75589748 title "Cloning, nucleotide sequence, and expression of the DNA ligase-encoding gene from Thermus filiformis." @default.
- W75589748 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9749531" @default.
- W75589748 hasPublicationYear "1998" @default.
- W75589748 type Work @default.
- W75589748 sameAs 75589748 @default.
- W75589748 citedByCount "2" @default.
- W75589748 crossrefType "journal-article" @default.
- W75589748 hasAuthorship W75589748A5051265114 @default.
- W75589748 hasAuthorship W75589748A5084232009 @default.
- W75589748 hasConcept C104317684 @default.
- W75589748 hasConcept C134459356 @default.
- W75589748 hasConcept C13575015 @default.
- W75589748 hasConcept C140248907 @default.
- W75589748 hasConcept C153911025 @default.
- W75589748 hasConcept C167625842 @default.
- W75589748 hasConcept C175114707 @default.
- W75589748 hasConcept C181199279 @default.
- W75589748 hasConcept C185592680 @default.
- W75589748 hasConcept C19924922 @default.
- W75589748 hasConcept C203284983 @default.
- W75589748 hasConcept C25602115 @default.
- W75589748 hasConcept C2776027139 @default.
- W75589748 hasConcept C27911776 @default.
- W75589748 hasConcept C2908738455 @default.
- W75589748 hasConcept C47558249 @default.
- W75589748 hasConcept C49105822 @default.
- W75589748 hasConcept C52182516 @default.
- W75589748 hasConcept C552990157 @default.
- W75589748 hasConcept C55493867 @default.
- W75589748 hasConcept C86803240 @default.
- W75589748 hasConcept C90583042 @default.
- W75589748 hasConceptScore W75589748C104317684 @default.
- W75589748 hasConceptScore W75589748C134459356 @default.
- W75589748 hasConceptScore W75589748C13575015 @default.
- W75589748 hasConceptScore W75589748C140248907 @default.
- W75589748 hasConceptScore W75589748C153911025 @default.
- W75589748 hasConceptScore W75589748C167625842 @default.
- W75589748 hasConceptScore W75589748C175114707 @default.
- W75589748 hasConceptScore W75589748C181199279 @default.
- W75589748 hasConceptScore W75589748C185592680 @default.
- W75589748 hasConceptScore W75589748C19924922 @default.
- W75589748 hasConceptScore W75589748C203284983 @default.
- W75589748 hasConceptScore W75589748C25602115 @default.
- W75589748 hasConceptScore W75589748C2776027139 @default.
- W75589748 hasConceptScore W75589748C27911776 @default.
- W75589748 hasConceptScore W75589748C2908738455 @default.
- W75589748 hasConceptScore W75589748C47558249 @default.
- W75589748 hasConceptScore W75589748C49105822 @default.
- W75589748 hasConceptScore W75589748C52182516 @default.
- W75589748 hasConceptScore W75589748C552990157 @default.
- W75589748 hasConceptScore W75589748C55493867 @default.
- W75589748 hasConceptScore W75589748C86803240 @default.
- W75589748 hasConceptScore W75589748C90583042 @default.
- W75589748 hasIssue "4" @default.
- W75589748 hasLocation W755897481 @default.
- W75589748 hasOpenAccess W75589748 @default.
- W75589748 hasPrimaryLocation W755897481 @default.
- W75589748 hasRelatedWork W1992278173 @default.
- W75589748 hasRelatedWork W2050426655 @default.
- W75589748 hasRelatedWork W2068109586 @default.
- W75589748 hasRelatedWork W2073788366 @default.
- W75589748 hasRelatedWork W2080784555 @default.
- W75589748 hasRelatedWork W2105427113 @default.
- W75589748 hasRelatedWork W2112250293 @default.
- W75589748 hasRelatedWork W2162737386 @default.
- W75589748 hasRelatedWork W3027172257 @default.
- W75589748 hasRelatedWork W75589748 @default.
- W75589748 hasVolume "8" @default.
- W75589748 isParatext "false" @default.
- W75589748 isRetracted "false" @default.
- W75589748 magId "75589748" @default.
- W75589748 workType "article" @default.