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- W757777230 abstract "Three kinds of serine protease inhibitors, members of the Bowman-Birk trypsin inhibitor, were purified from Dolichos lablab seeds and named Dolichos protease inhibitor 1,. 2 and 3 (DI-1, DI-2 and DI-3), respectively Each inhibitor showed a single band with gel mobility at around 15.9, 12.1 and 14.6 kDa on 20% SDS-PAGE under reducing conditions. To characterize inhibitory specificity, the inhibition constant (Ki) for these inhibitors was measured against several known serine proteases. All three Dolichos protease inhibitors (DI-1, DI-2 and DI-3) inhibited the activity of trypsin and plasmin, but had no effect on thrombin and kallikrein (either for human plasma kavikrein or for porcine pancreas kallikrein). DI-1 inhibited chymotrypsin most effectively (Ki = 3.6×10^(-9) M), while DI-2 displayed inhibitory activity for porcine pancreatic elastase (Ki = 6.2×10^(-8) M). Pre-treatment of the 33 ㎎/㎏ of DI-mixture (active fractions from C,8 open column chromatography that included DI-1, DI-2 and DI-3) inhibited the induction of pseudomonal elastase-induced septic hypotension and prevented an increase in bradykinin generation in pseudomonal elastase-treated guinea pig plasma. Also, the increase of kallikrein activity, by injection of pseudomonal elastase, was inhibited by the pretreahnent of th:e DI-mixture in a guinea pig. Since the DI-mixture had no inhibitory effect on kallikrein activity when Z-Phe-ArgMCA was used as a substrate in vitro, its inhibitory activity in the pseudomonal elastase-induced septic hypotension model might not be due to a direct inhibition of plasma kallikrein in the activation cascade of the Hageman factor and prekallikrein system. These results suggest that the Doliehos DI-mixture might be used as an inhibitor in pathogenic bacterial protease-induced septic shock." @default.
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- W757777230 date "2000-01-01" @default.
- W757777230 modified "2023-09-24" @default.
- W757777230 title "Purification and Characterization of Serine Protease Inhibitors from Dolichos lablab Seeds; Prevention Effects on Pseudomonal Elastase-Induced Septic Hypotension" @default.
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