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• W75797419 abstract "The transversal distribution of phosphatidylcholine, phosphatidylethanolamine, and cardiolipin in the inner membrane of beef heart mitochondria was investigated. Highly purified “right-side-out” (mitoplasts) and “inside-out” (submitochondrial particles) membranes were used, permitting the independent study of the phospholipid composition on either half of the membrane. Three independent, and partially complementary, methods were employed: (a) chemical labeling with fluorescamine; (b) phospholipase AZ treatment; (c) immunoreaction with anticardiolipin antibodies. (a) 41 and 61% of the total phosphatidylethanolamine in mitoplasts and submitochondrial particles, respectively, were labeled with fluorescamine. With membranes solubilized by deoxycholate about 95% of the total phosphatidylethanolamine was labeled. (b) Phospholipase AZ (EC 3.1.1.4) from NC& nuju venom hydrolyzed 73% of the total phosphatidylcholine and 39% of the total phosphatidylethanolamine in mitoplasts, and 30% of phosphatidylcholine and 63% of phosphatidylethanolamine in submitochondrial particles, provided the enzyme was purified according to Deems and Dennis (Deems, R. A., and Dennis, E. A. (1975) J. Biol. Chem. 250,9008-9012). After solubilizing the membranes with Triton X-100, more than 90% of the phospholipids were hydrolyzed. The rate of hydrolysis was fast in the case of phosphatidylethanolamine and phosphatidylcholine but slow in the case of cardiolipin. After 60 min, 20 and 60% of the total cardiolipin was hydrolyzed in mitoplasts and submitochondrial vesicles, respectively. (c) The immunoreaction with cardiolipin antibodies indicated cardiolipin to be about 3 times more abundant on the matrix side of the membrane. From the three approaches applied, the following phospholipid composition of the inner membrane of heart mitochondria was obtained: tin the cytoplasmic side (mitoplasts) the composition was 28% phosphatidylcholine, 13% phosphatidylethanolamine, 6% cardiolipin; on the matrix side (submitochondrial particles) the composition was 11% phosphatidylcholine, 21% phosphatidylethanolamine, 18% cardiolipin." @default.
• W75797419 created "2016-06-24" @default.
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• W75797419 date "1979-06-01" @default.
• W75797419 modified "2023-10-18" @default.
• W75797419 title "Asymmetric distribution of phospholipids in the inner membrane of beef heart mitochondria." @default.
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• W75797419 doi "https://doi.org/10.1016/s0021-9258(18)50596-2" @default.
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