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• W758406075 abstract "The issues that most affect the water quality of rivers and lakes are associated with discharges in these environments, especially those of domestic and industrial sewage inadequately or not treated. These effluents may contain hazardous chemical compounds for their ability to induce cytotoxic and genotoxic effects to the biota exposed. In this sense, the present study aimed to determine the potential cytotoxic, genotoxic and mutagenic of chemicals present in the waters of the ribeirao Tatu, Limeira / SP, from illegal discharges from small industries and domestic sewage through the test of chromosomal aberrations (CAs) and micronucleus (MN) in Allium cepa and comet assay in mammalian cells. Five seasonally collections were performed at five different places of the river in 2008 and 2009. The physical-chemical analysis of water samples showed a large degradation in water quality of the stream, resulting from discharges of municipal and industrial wastewater, storm water and contaminated runoff from agriculture. The data on A. cepa bioassay show that the droughts are the most critical because the answers led to more severe induction of CAs and MN in the test organism used. Concerning the comet assay with HTC cells, water samples collected during the period of strong rain, had higher genotoxicity, especially at points of most impacted by the receipt of effluents, due possibly to the carrying of contaminants to riverbed, sponsored by runoff from rainfall. Key-words: water resources contamination, Allium cepa, HTC cells, environmental biomonitoring. SUMARIO Pagina 1. INTRODUCAO 8 2. REVISAO DA LITERATURA 2.1. Contaminacao de recursos hidricos 10 2.2. Caracterizacao da area de estudo 12 2.3. Allium cepa como sistema-teste para o monitoramento ambiental 14 2.4. Celulas de mamiferos, mantidas em cultura, usadas como sistema-teste 16 2.5. Teste ACs 17 2.6. Ensaio do cometa 19 3. OBJETIVOS 22 4. MATERIAL E METODOS 4.1. Localizacao da area de estudo e coleta das amostras de agua 23 4.2. Material biologico 26 4.3. Analise fisico-quimica das amostras de agua 26 4.4. Teste de ACs e do MN em celulas meristematicas de A. cepa 26 4.5. Teste do MN em celulas F1 de A. cepa 27 4.6. Ensaio do cometa com cultura de celulas de mamiferos (celulas HTC) 28 5. RESULTADOS 5.1. Analise dos parâmetros fisico-quimicos 30 5.2. Artigo 1: O uso de cultura de celulas de mamiferos em avaliacoes da contaminacao de recursos hidricos 38 5.3. Artigo 2: Avaliacao da toxicidade das aguas do ribeirao Tatu, regiao de Limeira/SP, por meio do sistema-teste Allium cepa 63 5.4. Artigo 3: Avaliacao da genotoxicidade de aguas impactadas por efluentes domesticos e industriais de uma regiao altamente industrializada do Estado de Sao Paulo-Brasil, por meio do ensaio do cometa em celulas HTC 89 6. CONSIDERACOES FINAIS 108 7. REFERENCIAS BIBLIOGRAFICAS 110" @default.
• W758406075 created "2016-06-24" @default.
• W758406075 creator A5045769449 @default.
• W758406075 date "2011-03-17" @default.
• W758406075 modified "2023-09-30" @default.
• W758406075 title "Avaliação dos potenciais citotóxico, genotóxico e mutagênico das águas do Ribeirão Tatu, região de Limeira/SP, após o recebimento de efluentes urbanos" @default.
• W758406075 doi "https://doi.org/10.5016/dt000618429" @default.
• W758406075 hasPublicationYear "2011" @default.
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