Matches in SemOpenAlex for { <https://semopenalex.org/work/W758524053> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W758524053 endingPage "41" @default.
- W758524053 startingPage "32" @default.
- W758524053 abstract "CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatability. Interestingly, when cspA mRNA encoding a premature nonsense codon was overexpressed at low temperature, cell growth was completely inhibited. This phenotype was termed LACE (the low temperature-dependent antibiotic effect of truncated cspA expression), and this lethality resulted from exclusive stalling of most ribosomes on mutant cspA mRNAs. In a previous study, we demonstrated that overexpression of the ATP-dependent DNA helicases, UvrD and DinG, suppressed the lethality and ribosome stalling caused by mutant cspA mRNA. In the present study, we attempted to elucidate how these two DNA helicases help recover normal growth under LACE condition. Interestingly, we found that UvrD and DinG appeared to have an ability to down-regulate the replication of pUC-based high copy plasmid. In plasmid copy number tests, the amount of pUC-based plasmid encoding mutant cspA was reduced by 3–10-fold when either UvrD or DinG was expressed. Through a β-galactosidase activity assay, we also confirmed that expression of the lacZα gene inserted into the pUC-based plasmid was significantly reduced due to down-regulation of plasmid replication. Our findings imply that UvrD and DinG, known as non-replicative helicases, play a novel role in the regulation of ColE1-like plasmid replication." @default.
- W758524053 created "2016-06-24" @default.
- W758524053 creator A5006561075 @default.
- W758524053 creator A5036440303 @default.
- W758524053 creator A5040687350 @default.
- W758524053 creator A5047536712 @default.
- W758524053 date "2015-09-01" @default.
- W758524053 modified "2023-09-23" @default.
- W758524053 title "Inhibitory effect of UvrD and DinG on the replication of ColE1-derived plasmids in Escherichia coli" @default.
- W758524053 cites W1498020501 @default.
- W758524053 cites W1585911315 @default.
- W758524053 cites W1672694014 @default.
- W758524053 cites W1970386271 @default.
- W758524053 cites W2002142911 @default.
- W758524053 cites W2014571977 @default.
- W758524053 cites W2017682047 @default.
- W758524053 cites W2028198542 @default.
- W758524053 cites W2028622989 @default.
- W758524053 cites W2030293852 @default.
- W758524053 cites W2042891873 @default.
- W758524053 cites W2043993012 @default.
- W758524053 cites W2067257071 @default.
- W758524053 cites W2069083720 @default.
- W758524053 cites W2071434125 @default.
- W758524053 cites W2081622718 @default.
- W758524053 cites W2081859323 @default.
- W758524053 cites W2082063129 @default.
- W758524053 cites W2082090331 @default.
- W758524053 cites W2089737855 @default.
- W758524053 cites W2132656109 @default.
- W758524053 cites W2139913518 @default.
- W758524053 cites W2141653906 @default.
- W758524053 cites W2146349577 @default.
- W758524053 cites W2150879617 @default.
- W758524053 cites W2152234140 @default.
- W758524053 cites W2152531512 @default.
- W758524053 cites W2159704485 @default.
- W758524053 cites W2160084442 @default.
- W758524053 cites W2161923400 @default.
- W758524053 cites W2164577353 @default.
- W758524053 cites W2172916891 @default.
- W758524053 doi "https://doi.org/10.1016/j.plasmid.2015.06.002" @default.
- W758524053 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26143370" @default.
- W758524053 hasPublicationYear "2015" @default.
- W758524053 type Work @default.
- W758524053 sameAs 758524053 @default.
- W758524053 citedByCount "1" @default.
- W758524053 countsByYear W7585240532021 @default.
- W758524053 crossrefType "journal-article" @default.
- W758524053 hasAuthorship W758524053A5006561075 @default.
- W758524053 hasAuthorship W758524053A5036440303 @default.
- W758524053 hasAuthorship W758524053A5040687350 @default.
- W758524053 hasAuthorship W758524053A5047536712 @default.
- W758524053 hasConcept C104317684 @default.
- W758524053 hasConcept C143065580 @default.
- W758524053 hasConcept C153911025 @default.
- W758524053 hasConcept C161223559 @default.
- W758524053 hasConcept C22744801 @default.
- W758524053 hasConcept C2777014509 @default.
- W758524053 hasConcept C54355233 @default.
- W758524053 hasConcept C547475151 @default.
- W758524053 hasConcept C67705224 @default.
- W758524053 hasConcept C73573662 @default.
- W758524053 hasConcept C86803240 @default.
- W758524053 hasConceptScore W758524053C104317684 @default.
- W758524053 hasConceptScore W758524053C143065580 @default.
- W758524053 hasConceptScore W758524053C153911025 @default.
- W758524053 hasConceptScore W758524053C161223559 @default.
- W758524053 hasConceptScore W758524053C22744801 @default.
- W758524053 hasConceptScore W758524053C2777014509 @default.
- W758524053 hasConceptScore W758524053C54355233 @default.
- W758524053 hasConceptScore W758524053C547475151 @default.
- W758524053 hasConceptScore W758524053C67705224 @default.
- W758524053 hasConceptScore W758524053C73573662 @default.
- W758524053 hasConceptScore W758524053C86803240 @default.
- W758524053 hasFunder F4320321284 @default.
- W758524053 hasFunder F4320322120 @default.
- W758524053 hasFunder F4320322349 @default.
- W758524053 hasLocation W7585240531 @default.
- W758524053 hasLocation W7585240532 @default.
- W758524053 hasOpenAccess W758524053 @default.
- W758524053 hasPrimaryLocation W7585240531 @default.
- W758524053 hasRelatedWork W1508194938 @default.
- W758524053 hasRelatedWork W1563778277 @default.
- W758524053 hasRelatedWork W2014153860 @default.
- W758524053 hasRelatedWork W2016433237 @default.
- W758524053 hasRelatedWork W2027466503 @default.
- W758524053 hasRelatedWork W2052557924 @default.
- W758524053 hasRelatedWork W2054191026 @default.
- W758524053 hasRelatedWork W2154162036 @default.
- W758524053 hasRelatedWork W2166198415 @default.
- W758524053 hasRelatedWork W2397228493 @default.
- W758524053 hasVolume "81" @default.
- W758524053 isParatext "false" @default.
- W758524053 isRetracted "false" @default.
- W758524053 magId "758524053" @default.
- W758524053 workType "article" @default.