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- W760154785 abstract "目的：建立TaqMan-MGB技术实时定量检测人IL-6 mRNA的方法，并用于检测人外周血单个核细胞在牛膝多糖诱导下IL-6 mRNA的表达。方法：(1)抽取人外周静脉血，EDTA抗凝，用淋巴细胞分离液分离外周血单个核细胞，用Trizol裂解液提取总RNA，将总RNA逆转录为cDNA，PCR扩增IL-6后纯化PCR产物，与pMD 18-T载体进行连接，转化宿主菌DH-5α。提取重组质粒DNA，作为实时荧光定量检测的标准品。建立TaqMan-MGB技术实时定量检测IL-6 mRNA的方法。(2)终浓度分别为0、100、200、400、800和1600mg/L的牛膝多糖作用于人外周血单个核细胞，定量检测IL-6 mRNA的表达。结果：(1)酶切分析、PCR扩增以及测序结果均证实IL-6 cDNA成功重组到pMD 18-T载体上。(2)TaqMan-MGB技术检测IL-6 mRNA线性范围广；灵敏度高；特异性好；重复性好。(3)牛膝多糖作用于人外周血单个核细胞后IL-6 mRNA表达增高。结论：TaqMan-MGB技术能较为精确地定量IL-6 mRNA。" @default.
- W760154785 created "2016-06-24" @default.
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- W760154785 date "2008-01-15" @default.
- W760154785 modified "2023-09-23" @default.
- W760154785 title "人IL-6 mRNA的实时定量检测法" @default.
- W760154785 hasPublicationYear "2008" @default.
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