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- W76141957 abstract "In this study, we investigated culture conditions of primary porcine hepatocytes for development of a hybrid artificial liver using polyurethane foam (PUF)/spheroid culture. Porcine hepatocytes in the pores of hydrophilic PUF only formed spheroids spontaneously within 1.5 days of culture, and maintained ammonia detoxification activity in Williams’ E medium supplemented with 10∼20% fetal bovine serum (FBS) for a week. The porcine hepatocyte spheroids in multi-channel (MC) PUF packed-bed module also expressed the highest activities of ammonia detoxification and albumin production at an immobilized cell density of 1.0×107 cells/cm3 module and at an intrachannel medium velocity range from 60 to 80 cm/min." @default.
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- W76141957 date "2006-03-11" @default.
- W76141957 modified "2023-09-27" @default.
- W76141957 title "Development of a Hybrid Artificial Liver Support System with Porcine Hepatocyte Spheroids for Preclinical Use" @default.
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- W76141957 doi "https://doi.org/10.1007/0-306-46865-4_47" @default.
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