Matches in SemOpenAlex for { <https://semopenalex.org/work/W761507970> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W761507970 endingPage "1016" @default.
- W761507970 startingPage "1009" @default.
- W761507970 abstract "Abietane diterpenes have attracted much attention because they display a wide range of biological activities, including antitumor activities. These compounds are the most diverse of the diterpenoids isolated from species of Plectranthus. Naturally occurring diterpene parvifloron D is the main phytochemical constituent of Plectranthus ecklonii. To examine the therapeutic potential of the plant, we evaluated whether parvifloron D displays cytotoxicity against human tumor cells. The cytotoxicity was analyzed by colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Apoptosis was evaluated by fluorescent microscopy, transmission electron microscopy, flow cytometric analysis of annexin V-FITC and propidium iodide-stained cells and DNA fragmentation. Protein expression and processing and release of mitochondrial proteins were analyzed by Western blot. Caspase activity was determined using colorimetric substrates. The membrane potential and intracellular reactive oxygen species were detected by flow cytometry. Parvifloron D displays strong cytotoxic properties against leukemia cells (HL-60, U-937, MOLT-3 and K-562) and in particular P-glycoprotein-overexpressing K-562/ADR cells, but has only weak cytotoxic effects on peripheral blood mononuclear cells (PBMCs). Overexpression of the protective mitochondrial proteins Bcl-2 and Bcl-xL did not confer resistance to parvifloron D-induced cytotoxicity. Growth inhibition of HL-60 cells that was triggered by parvifloron D was found to be caused by a rapid induction of apoptotic cell death. This apoptosis was prevented by the non-specific caspase inhibitor z-VAD-fmk, and by the selective caspase-9 inhibitor z-LEHD-fmk. Cell death induced by parvifloron D was found to be (i) associated with the dissipation of the mitochondrial membrane potential and the release of cytochrome c, (ii) amplified by inhibition of extracellular signal-regulated kinases (ERKs) 1/2 signaling and (iii) caused by a mechanism dependent on intracellular reactive oxygen species generation. Parvifloron D is a potent cytotoxic compound against several human tumor cells and also a fast and potent apoptotic inducer in leukemia cells." @default.
- W761507970 created "2016-06-24" @default.
- W761507970 creator A5001432282 @default.
- W761507970 creator A5012111165 @default.
- W761507970 creator A5015909622 @default.
- W761507970 creator A5019696976 @default.
- W761507970 creator A5053376200 @default.
- W761507970 creator A5066462755 @default.
- W761507970 date "2015-10-01" @default.
- W761507970 modified "2023-10-18" @default.
- W761507970 title "The abietane diterpenoid parvifloron D from Plectranthus ecklonii is a potent apoptotic inducer in human leukemia cells" @default.
- W761507970 cites W1965694039 @default.
- W761507970 cites W1975260763 @default.
- W761507970 cites W1977258127 @default.
- W761507970 cites W1979925461 @default.
- W761507970 cites W1979995768 @default.
- W761507970 cites W1990022495 @default.
- W761507970 cites W1996180957 @default.
- W761507970 cites W2006000887 @default.
- W761507970 cites W2017009693 @default.
- W761507970 cites W2017668985 @default.
- W761507970 cites W2022803736 @default.
- W761507970 cites W2028151158 @default.
- W761507970 cites W2049097879 @default.
- W761507970 cites W2089333447 @default.
- W761507970 cites W2136466466 @default.
- W761507970 cites W2151795172 @default.
- W761507970 cites W2153890808 @default.
- W761507970 cites W2172162633 @default.
- W761507970 cites W3113125785 @default.
- W761507970 doi "https://doi.org/10.1016/j.phymed.2015.06.013" @default.
- W761507970 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26407943" @default.
- W761507970 hasPublicationYear "2015" @default.
- W761507970 type Work @default.
- W761507970 sameAs 761507970 @default.
- W761507970 citedByCount "32" @default.
- W761507970 countsByYear W7615079702016 @default.
- W761507970 countsByYear W7615079702017 @default.
- W761507970 countsByYear W7615079702018 @default.
- W761507970 countsByYear W7615079702019 @default.
- W761507970 countsByYear W7615079702020 @default.
- W761507970 countsByYear W7615079702021 @default.
- W761507970 countsByYear W7615079702022 @default.
- W761507970 countsByYear W7615079702023 @default.
- W761507970 crossrefType "journal-article" @default.
- W761507970 hasAuthorship W761507970A5001432282 @default.
- W761507970 hasAuthorship W761507970A5012111165 @default.
- W761507970 hasAuthorship W761507970A5015909622 @default.
- W761507970 hasAuthorship W761507970A5019696976 @default.
- W761507970 hasAuthorship W761507970A5053376200 @default.
- W761507970 hasAuthorship W761507970A5066462755 @default.
- W761507970 hasConcept C109316439 @default.
- W761507970 hasConcept C153911025 @default.
- W761507970 hasConcept C154317977 @default.
- W761507970 hasConcept C185592680 @default.
- W761507970 hasConcept C190283241 @default.
- W761507970 hasConcept C202751555 @default.
- W761507970 hasConcept C206103511 @default.
- W761507970 hasConcept C2775934118 @default.
- W761507970 hasConcept C2780868391 @default.
- W761507970 hasConcept C31573885 @default.
- W761507970 hasConcept C43759708 @default.
- W761507970 hasConcept C55493867 @default.
- W761507970 hasConcept C86803240 @default.
- W761507970 hasConceptScore W761507970C109316439 @default.
- W761507970 hasConceptScore W761507970C153911025 @default.
- W761507970 hasConceptScore W761507970C154317977 @default.
- W761507970 hasConceptScore W761507970C185592680 @default.
- W761507970 hasConceptScore W761507970C190283241 @default.
- W761507970 hasConceptScore W761507970C202751555 @default.
- W761507970 hasConceptScore W761507970C206103511 @default.
- W761507970 hasConceptScore W761507970C2775934118 @default.
- W761507970 hasConceptScore W761507970C2780868391 @default.
- W761507970 hasConceptScore W761507970C31573885 @default.
- W761507970 hasConceptScore W761507970C43759708 @default.
- W761507970 hasConceptScore W761507970C55493867 @default.
- W761507970 hasConceptScore W761507970C86803240 @default.
- W761507970 hasFunder F4320335322 @default.
- W761507970 hasIssue "11" @default.
- W761507970 hasLocation W7615079701 @default.
- W761507970 hasLocation W7615079702 @default.
- W761507970 hasOpenAccess W761507970 @default.
- W761507970 hasPrimaryLocation W7615079701 @default.
- W761507970 hasRelatedWork W1968505653 @default.
- W761507970 hasRelatedWork W2008479527 @default.
- W761507970 hasRelatedWork W2033803187 @default.
- W761507970 hasRelatedWork W2061489521 @default.
- W761507970 hasRelatedWork W2090535286 @default.
- W761507970 hasRelatedWork W2095973419 @default.
- W761507970 hasRelatedWork W2167141699 @default.
- W761507970 hasRelatedWork W2328524531 @default.
- W761507970 hasRelatedWork W2337084509 @default.
- W761507970 hasRelatedWork W2357971202 @default.
- W761507970 hasVolume "22" @default.
- W761507970 isParatext "false" @default.
- W761507970 isRetracted "false" @default.
- W761507970 magId "761507970" @default.
- W761507970 workType "article" @default.