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- W761640705 abstract "To construct a suppression subtractive hybridization(SSH) cDNA library of male Haemaphysalis longicornis and to screen the differentially expressed gene in the partially-engorged condition,the cDNA library of partially-engorged male H.longicornis was constructed in the clone vector of pGEM-T Easy by SSH.The sequencing and bioinformatics analyses were conducted afterwards.The quality of the library was evaluated by RT-PCR using the first strand of partially-engorged male H.longicornis cDNA newly synthesized as the template.Eighteen expressed sequence tags(ESTs) were obtained from the SSH cDNA library and BlastX analysis revealed that the putative proteins encoded by these ESTs had similarity to proteins reported before,with most of which were functional proteins,such as signal transduction,tissue morphogenesis,transcriptional regulation and carbohydrate metabolism proteins.These proteins are valuable to the ticks to adapt the changes of physiology after feeding.The result of RT-PCR revealed that 3 of 5 ESTs from the library were differentially expressed in the partially-engorged condition.The results suggested that the SSH cDNA library of male H.longicornis was constructed successfully,and some potential functional ESTs were obtained." @default.
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- W761640705 date "2009-01-01" @default.
- W761640705 modified "2023-09-23" @default.
- W761640705 title "Construction and analysis of SSH cDNA library of male Haemaphysalis longicornis" @default.
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