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- W761701840 abstract "【Objective】The study was done in order to provide a theoretical basis for genetic improvement by comparing the genetic diversity of the Neosinocalamus affinis from different regions in Sichuan province.【Method】Neosinocalamus affinis from the 12 typical regions of Sichuan was studied by using 29 randomly amplified polymorphic DNA(RAPD) and 12 inter-simple sequence repeat(ISSR) primers.The genetic diversity was studied by using RAPD and ISSR amplification markers and the specific band was cloned.【Result】14 RAPD primers were selected,168 bands amplified,and 142 bands polymorphic,of which the percentage of polymorphic bands(PPB) was 84.52% for RAPDs.8 ISSR primers were selected,73 bands amplified,and 56 bands polymorphic,of which the percentage of polymorphic bands(PPB) was 76.71%.The genetic distance ranged from 0.140 4 to 0.570 1 for RAPDs and 0.071 0 to 0.706 9 for ISSRs.The length of the special band of Neosinocalamus affinis from Qingshen county in Meishan city(C-MSQS) by primer OPAA-04 was 570 bp and the amplified fragment was related to cytochrome c oxidase subunit 1.【Conclusion】The Neosinocalamus affinis of Sichuan germplasm resources had a high genetic diversity." @default.
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- W761701840 date "2009-01-01" @default.
- W761701840 modified "2023-09-27" @default.
- W761701840 title "Assessment of genetic diversity in Neosinocalamus affinis from different regions of Sichuan province" @default.
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