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- W763232555 abstract "【Objective】 To improve the immobilization effect of neutral proteinase,the immobilization condition and enzyme properties of neutral proteinase were studied with resin D101.【Method】 The Absorption method was used to detect immobilization effect under different temperatures,pH and enzyme concentrations.The Km,thermal stability and pH stability of immobilized and soluble enzyme were studied.【Result】 The optimized results were achieved with 150 U/g of the amount of neutral proteinase to resin in weight,coupling temperature at 40 ℃ and immobilizing time of 120 min.The maximum yield of immobilized enzyme was 70%.The optimal temperature and optimal pH of immobilized and soluble enzyme were at 46 and 40 ℃,7.4 and 7.2,respectively.The Km and Vmax of immobilized and soluble enzyme were 0.22 mg/mL,0.39 mL/min and 0.20 mg/mL,0.21 mL/min,respectively.【Conclusion】 Compared with free enzyme,the enzyme activity,enzyme recovery and stability of the immobilized enzyme were significantly increased." @default.
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- W763232555 date "2009-01-01" @default.
- W763232555 modified "2023-09-23" @default.
- W763232555 title "Study on immobilized condition optimization and properties of neutral proteinase with resin D101." @default.
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