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- W763702014 abstract "目的：观察健骨颗粒含药血清对成骨样细胞增殖及PCNA、Cyclin D1 mRNA表达的影响。方法：制备健骨颗粒含药血清和生理盐水血清,分别干预体外培养的成骨样细胞ROS17/2.8,MTT法检测细胞增殖,流氏细胞术检测细胞周期,荧光定量PCR检测PCNA、Cyclin D1 mRNA表达。结果：与同浓度生理盐水血清组相比,中药血清组10%-30%浓度促细胞增殖均有显著性差异（P〈0.05）,其中浓度为20%时,两组间有非常显著性差异（P〈0.01）;中药血清组G0/G1期细胞比例明显低于同期生理盐水血清组,有非常显著性差异（P〈0.01）。G2/M期、S期细胞比例及PI在干预48h时均高于同期生理盐水血清组,具非常显著性差异（P〈0.01）。中药血清组较理盐水血清组PCNA、Cyclin D1 mRNA表达升高,组间有显著性差异（P〈0.05）。结论：健骨颗粒含药血清能促进成骨样细胞的增殖、提高PCNA、Cyclin D1 mRNA表达。" @default.
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- W763702014 date "2012-01-01" @default.
- W763702014 modified "2023-09-24" @default.
- W763702014 title "健骨颗粒含药血清对成骨样细胞增殖及PCNA、Cyclin D1 mRNA表达的影响" @default.
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