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• W763910405 abstract "Canine pyometra is a disease characterized by the inflammation of the uterus with accumulation of purulent discharge, affecting mainly adult animals. Occurs the luteus phase of the estrus cycle result of hormone alterations and generally is associated with bacterial infections. It is recognized as one of the main causes of disease and death the bitch and Escherichia coli is the major pathogen associated with this disease. The aim of this study was to research, isolation and identification of Escherichia coli strains from intrauterine contents, mouth and feces of bitches diagnosed with pyometra, studying the prevalence of uropathogenic virulence factors genes strains isolated, still testing the genetic similarity among strains isolated from intrauterine contents and mouth and evaluate the susceptibility in vitro of the isolated bacteria to 12 antimicrobial drugs. Seventy E. coli strains, 25 from intrauterine contents, 26 from mouth and 19 from feces isolated from six bitches were examined by PCR. Among the strains 67 (95.7%) were positive for fim, 19 (27.1%) were positive for iss, 18 (25.7%) were positive for hly, 13 (18.5%) were positive for iuc and 12 (17.1%) were positive for usp. Multiple antimicrobial resistance was detected for cephalothin (68.0%), nalidixic acid (56.0%) and ampicillin (56.0%) among the pyomera E. coli isolates. Multidrug resistance was found 12 (48.0%) of the isolates from pyometra, 22 (84.6%) of the isolates from mouth and 14 (73.6%) of the isolates from feces. It must be considered that dogs are animals that are living close proximity to human and have an important role the transmission of E. coli to others animals or human, highlighting the major risks facing the large number of UVF and multidrug resistance found the E.coli strains recovered from the pet samples. Three animals showed strong similarity among E. coli strains isolated from intrauterine contents and mouth, through the analysis of genetic diversity performed by use of the technique REP-PCR, ERIC-PCR and BOXPCR. Multiple antimicrobial resistance was detected for cephalothin (67.1%), ampicillin (65.7%), tetracycline (61,4%) and nitrofurantoin (58,5%) among the E. coli isolates. Multidrug resistance was found 12 (48.0%) of the isolates from intrauterine contents, 22 (84.6%) of the isolates from mouth and 14 (73.6%) of the isolates from feces. It must be considered that dogs are animals that are living close proximity to human and have an important role the transmission of E. coli to others animals or human, highlighting the major risks facing the large number of UVF and multidrug resistance found the E.coli strains recovered from the pet samples." @default.
• W763910405 created "2016-06-24" @default.
• W763910405 creator A5025683778 @default.
• W763910405 date "2013-07-26" @default.
• W763910405 modified "2023-09-23" @default.
• W763910405 title "Diversidade genética, fatores de virulência e perfis de susceptibilidade a antimicrobianos de isolados de Escherichia coli provenientes do útero, da boca e das fezes de cadelas com piometra" @default.
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