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- W76398514 abstract "This research was designed to characterize an inhibitory effect of acetic acid on the activity of glycosidases expressed in Caco-2 cells. Cells were cultured in a medium containing 5 mM acetic acid for 15 days, and meantime the activity of sucrase was monitored. On the 3rd day in culture the activity of sucrase was approximately 3 μ mo1/hr/mg protein. This activitywasnot significantly different from that in cells cultured without acetic acid (control). There after, in control cells, the activity of sucrase increasedandreached 12 μmo1/hr/mg protein on the 15th day.However, the activity of sucrase in acetic acid treated cells barely increased and reached 4.7 μmo1/hr/mg protein on the 15th day. The activity of sucrase was suppressed in a dose dependent manner. We measured the activity of glycosidases and other hydrolases after 15-day treatment with 5 mM acetic acid. The activity of sucrase and maltase was suppressed to the level of 40 to 50% of the control and the activity of trehalase and lactase was drastically decreased to less than 10% of the control.However, the activity of alkaline phosphatase (ALP), aminopeptidase-N (AP-N), dipeptidylpeptidase-IV DPP-1V) and γ-glutamyltrans peptidase (γ-GTP) which are also present in the intestinal brush border membrane was not affected by acetic acid. Acetic acid treatment did not affect either cell growth or cell viability. To understand mechanisms underlying this suppression of glycosidase activity by acetic acid, we performed northern and western analyses of sucrase. Neither mRNA nor protein level of sucrase was decreased by treatment with 5 mM acetic acid for 15 days. These results suggest that acetic acid may suppress the increase of glycosidase activity during the growth of Caco-2 cells by blocking a biological step followingtranscriptionandtranslation,suchastraffickingof denovosynthesized glycosidases and their incorporation into the brush border membrane." @default.
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- W76398514 date "2005-11-30" @default.
- W76398514 modified "2023-09-23" @default.
- W76398514 title "Acetic Acid Suppresses the Increase of Glycosidase Activity During Culture of Caco-2 Intestinal Epithelial Cells" @default.
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- W76398514 doi "https://doi.org/10.1007/0-306-46869-7_63" @default.
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