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- W765729209 abstract "Pluripotent stem cells (PSCs) offer an unlimited source of progenitors for the derivation of vascular cells, which can then be engineered to recreate functional vasculature and advance regenerative medicine. Previous methods to derive endothelial cells (ECs), which make up the inner lining of blood vessels, involve co-culture with mouse cells, which is not clinically relevant, or embryoid body formation, which can yield inconsistent results. To overcome these limitations, we have developed a feeder-free, 2D protocol to differentiate human PSCs, i.e. human embryonic stem cells and induced pluripotent stem cells, toward endothelial lineages. Results indicate that our hPSC niche generates cells expressing early endothelial markers, which upon sorting and re-culture, can mature into ECs. The differentiation products, analyzed by RT-PCR, flow cytometry, immunocytochemistry, and culture on Matrigel, demonstrate enrichment of EC-specific genes and proteins and functional capillary formation upon addition of vascular endothelial growth factor. These findings reveal that control over microenvironmental cues with appropriate signaling molecules is able to robustly produce critical cells of the vasculature, which may in turn serve as novel therapies for vascular diseases or be incorporated into engineered tissue. This research was funded by the March of Dimes." @default.
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- W765729209 date "2011-04-01" @default.
- W765729209 modified "2023-10-16" @default.
- W765729209 title "Derivation of endothelial cells from pluripotent stem cells in a feeder‐free, two‐dimensional niche" @default.
- W765729209 doi "https://doi.org/10.1096/fasebj.25.1_supplement.1043.12" @default.
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