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- W765754286 abstract "Canine distemper virus (CDV) is a member of genus Morbillivirus and familyParamyxoviridae that have a negative single-strand RNA genome (Appel, M.J.G., 1987). Caninedistemper is a multi-systemic viral disease of dogs characterized by a wide variety of clinicalmanifestations and lesions, and several other protozoal, bacterial and viral infections as well as toxinpoisoning mimic distemper symptoms (Beineke et al, 2009; Green & Appel, 1998). Therefore, thesigns are not always suggestive for canine distemper suspicion, and the etiological diagnosis need tobe performed. The most used methods of CDV diagnosis are ELISA, direct and indirectimmunofluorescent antibody tests, seroneutralisation, and immunofluorescent techniques to detectviral antigen were commercially available, and the veterinary laboratories usually perform them(Green & Appel, 1998). Unfortunately, the most of these techniques have their diagnostic limitationand sometimes can lead to false positive results. Thirty cases of dogs, different mixed breeds and agedless than one year were investigated for CDV infection by ELISA IgG (ImmunoComb®, Biogal,Israel), ELISA IgM (ImmunoComb®, Biogal, Israel), Immunochromatographic CDV-Ag test (CDVIC, Agrolabo, Italy), and reverse transcription polymerase chain reaction (RT-PCR). The blood serumsamples were collected from unvaccinated dogs with nervous manifestations and a history of diseaseup to one month. Eight (8/30) samples were IgM and IgG positive, fourteen (14/30) samples were onlyIgG positive, all immunochromatographic antigenic tests and RT-PCR were negative. In our opinion,the presence of CDV-antibodies leads to false-negative results for the antigenic and genomic viraltests. These results suggest that blood serum samples from dogs with nervous manifestations are notuseful for antigenic tests and RT-PCR." @default.
- W765754286 created "2016-06-24" @default.
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- W765754286 date "2010-01-01" @default.
- W765754286 modified "2023-09-23" @default.
- W765754286 title "From the Clinical to the Molecular Diagnosis of Canine Distemper Disease" @default.
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- W765754286 doi "https://doi.org/10.15835/buasvmcn-vm:67:2:6023" @default.
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