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- W76579573 abstract "Ultraviolet (UV) light causes DNA damage. The most common form of UV lesion found in DNA is the cis - syn pyrimidine dimer (CPD) in which adjacent pyrimidines on a DNA strand undergo a 2 + 2 photocycloaddition to generate a cyclobutane ring joining the two rings across the 5- and 6-carbon atoms of the pyrimidine ring. The structure of a thymidine–thymidine CPD lesion has been solved most recently by x-ray crystallography for a dodecamer duplex. Transient absorption spectra of photolyase with and without substrate are obtained in the visible region of the spectrum with subpicosecond time resolution. The spectrum of the flavosemiquinone is observed clearly for the first time, establishing without a doubt that photolyase functions via photo-induced electron transfer, leading to reductive cleavage of the CPD lesion. The decay of this state by charge recombination to the initial reduced flavin is also observed." @default.
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- W76579573 date "2006-01-01" @default.
- W76579573 modified "2023-09-24" @default.
- W76579573 title "Intermediates in the ultrafast repair of DNA by DNA photolyase" @default.
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- W76579573 doi "https://doi.org/10.1016/b978-044452821-6/50048-4" @default.
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