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• W767092216 abstract "the polymorphism of erythrocyte antigens in 12 blood group systems was tested in 744 red-andWhite cattle from south and south-west poland. microsatellite dna polymorphism was analysed using 128 blood samples taken from animals randomly selected from this group. the polymorphism of the markers studied was determined based on 149 alleles identified in 12 blood group systems and 79 alleles in 11 microsatellite sequences. the highest polymorphism was found in the a, b, c and s blood group systems, in which h and pic values ranged from 0.655 and 0.591 in the s system to 0.947 and 0.929 in the b system. in the f, J, l, m, Z, n’, r’ and t’ systems, h and pic values were less than 0.5. growing demand for high quality animal products and the search for quicker and more reliable methods of livestock identification have increased the scope of recent animal genetic variability studies with dna marker analysis. microsatellite sequences, 1–5 nucleotide sequences, and dna tandem repeats, also known as str (short tandem repeats) have found the widest application. the analysed microsatellite markers (tgla227, bm2113, tgla53, eth10, sps115, tgla126, tgla122, inra23, eth3, eth225, bm1824) were characterized by high polymorphism of the material studied. polymorphic information content (pic) estimates exceeded 0.7 for the tgla227, bm2113, tgla53, tgla122, inra23 and eth225 loci. for the other loci, pic exceeded 0.5 and h ranged from 0.597 (sps115) to as much as 0.863 (tgla53). the highest polymorphism was characteristic of the tgla53 marker, for which pic was 0.849 and h was 0.863. based on the estimated probability of exclusion (pec), it was found that incorrect parentage assignment can be excluded with 99.58% probability using blood group systems and with as much as 99.987% probability using microsatellite dna analysis. key words: cattle, erythrocyte antigens, dna microsatellites, parentage control The cattle parentage verification and identification system based on blood group tests has been used in Poland since the 1960s. Cattle pedigree data are confirmed based on erythrocyte antigens of 12 blood groups, which are determined using over 70 test sera. The probability of parentage exclusion based on the polymorphism of A. Radko and T. Rychlik 120 erythrocyte antigens exceeds 98% (Holm and Bendixen, 1996). In herds of cattle with an elevated inbreeding coefficient, homozygosity is increased and the gene pool limited. In this situation, paternity testing based on serological tests is often difficult or even impossible. In cases like these, microsatellite DNA sequences are increasingly used for pedigree verification in addition to blood groups (Heyen et al., 1997; Peelman et al., 1998; Radko et al., 2002). The current set of 11 highly polymorphic microsatellite markers estimates the probability of incorrect parentage assignment with 99.9% accuracy (Radko, 2008). The aim of the study was to compare the usefulness of blood group tests and microsatellite DNA sequences for parentage verification in a population of Polish Red-and-White cattle. material and methods The polymorphism of erythrocyte antigens in 12 blood group systems was tested in 744 Red-and-White cattle from south and south-west Poland. Microsatellite DNA polymorphism was analysed using 128 blood samples taken from animals randomly selected from this group. Erythrocyte antigens in the A, B, C, F, J, L, M, S, Z, N’, R’ and T’ systems were identified using 78 test reagents in the hemolytic test. All of the test reagents, obtained at the Department of Animal Immunoand Cytogenetics of the National Research Institute of Animal Production, were standardized in international comparison tests organized by the International Society for Animal Genetics (ISAG). DNA polymorphism was analysed using 11 microsatellite loci recommended by ISAG for parentage verification in cattle: TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225 and BM1824. These markers are part of the StockMarks for Cattle II kit (Applied Biosystems). Based on the genomic DNA isolated, the amplification of sequences from selected loci was performed by polymerase chain reaction (PCR) using fluorescently labelled primers. The markers were amplified in a single 11-plex reaction mixture. The amplification conditions followed the Protocol for Bovine II Version 2 (Mixed 11 Plex Kit) by Applied Biosystems. The PCR products obtained were analysed using an ABI 3130xl capillary sequencer (Applied Biosystems). Amplified DNA fragments of different length were electrophoresed in 7% denaturing polyacrylamide gel POP-7, in the presence of a GS500-ROX size standard and reference sample. The results of electrophoretic separation were analysed automatically using GeneMapper software. The frequency of individual blood group alleles and microsatellite sequences was used to calculate the degree of heterozygosity (H) and polymorphic information content (PIC). The probability of paternity exclusion (PE), taking into account the possibility of testing both parents, was calculated for each locus and separately for blood groups and microsatellite DNA markers. Blood groups and microsatellite markers in Polish Red-and-White cattle 121" @default.
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• W767092216 date "2009-01-01" @default.
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• W767092216 title "Use of blood group tests and microsatellite DNA markers for parentage verification in a population of Polish Red-and-White cattle." @default.
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