FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W768003421> ?p ?o ?g. }

• W768003421 abstract "Analytical separations form the bulk of experiments inboth research and industry. The choice of separation technique isgoverned by the characteristics of the analyte and purpose ofseparation. Miniaturization of chromatographic techniques enablesthe separation and purification of small volume samples that areoften in limited supply. Capillary electrophoresis andimmunoaffinity chromatography are examples of techniques that canbe easily miniaturized with minimum loss in separation efficiency.These techniques were used in the experiments presented in thisdissertation. Chapter 1 discusses the underlying principles ofcapillary electrophoresis and immunoaffinity chromatography. Inthe second chapter, the results from immunoaffinity chromatographyexperiments that utilized antibody-coated magnetic beads to purifyserine proteases and serine protease inhibitors (serpins) from A.gambiae hemolymph are presented and discussed. Serine proteases andserpins play a key role in the insect innate immunity system.Serpins regulate the activity of serine proteases by formingirreversible complexes with the proteases. To identify theproteases that couple to these serpins, protein A magnetic beadswere coated with SRPN2 antibody and then incubated with A. gambiaehemolymph. The antibody isolated both the free SRPN2 and theSRPN2-protease complex. The purified proteases were identified byESI-MS from as few as 25 insects. In Chapter 3, an integratedglass/PDMS hybrid microfluidic device was utilized for thetransportation and lysis of cells at a high throughput. Jurkatcells were labeled with 6-CFDA (an internal standard) and DAF-FM (aNO specific fluorophore). Laser-induced fluorescence (LIF)detection was utilized to detect nitric oxide (NO) from singleJurkat cells. The resulting electropherograms were used to studythe variation in NO production following stimulation withlipopolysaccharide (LPS). 3 h LPS-stimulation resulted in a twofold increase in NO production in both bulk and single cellanalysis. A comparison of bulk and single cell NO measurements wereperformed and the average NO production in single cells comparedwell to the increase measured at the bulk cell level. Chapter 4discusses the preliminary experiments with a T-shaped microfluidicdevice that exploit the property of poly(dimethylsiloxane) (PDMS)as an electroactive polymer (EAP), to enhance fluid mixing. EAPsdeform when placed in an electric field. A thin layer of PDMS wassandwiched between chrome electrodes, positioned on the horizontalarms of the T design, and the electrolyte-filled fluidic channel. Apotential difference across the PDMS layer caused it to shrink andstretch, thereby increasing the channel volume. The electrodes wereactuated at 180[degrees] out of phase and this caused the fluidstream in the vertical channel to fold and stretch resulting inenhanced contact surface area and shorter diffusion distances ofthe fluid, thereby improving mixing efficiency. All theexperiments presented in this dissertation demonstrate the…" @default.
• W768003421 created "2016-06-24" @default.
• W768003421 creator A5017323548 @default.
• W768003421 date "2013-04-29" @default.
• W768003421 modified "2023-09-25" @default.
• W768003421 title "Development of microanalytical methods for solving sample limiting biological analysis problems" @default.
• W768003421 cites W167776123 @default.
• W768003421 hasPublicationYear "2013" @default.
• W768003421 type Work @default.
• W768003421 sameAs 768003421 @default.
• W768003421 citedByCount "0" @default.
• W768003421 crossrefType "dissertation" @default.
• W768003421 hasAuthorship W768003421A5017323548 @default.
• W768003421 hasConcept C10390740 @default.
• W768003421 hasConcept C179247698 @default.
• W768003421 hasConcept C181199279 @default.
• W768003421 hasConcept C182220744 @default.
• W768003421 hasConcept C185592680 @default.
• W768003421 hasConcept C2776414213 @default.
• W768003421 hasConcept C2776714187 @default.
• W768003421 hasConcept C2777807008 @default.
• W768003421 hasConcept C2900643 @default.
• W768003421 hasConcept C43617362 @default.
• W768003421 hasConcept C55493867 @default.
• W768003421 hasConcept C57409179 @default.
• W768003421 hasConceptScore W768003421C10390740 @default.
• W768003421 hasConceptScore W768003421C179247698 @default.
• W768003421 hasConceptScore W768003421C181199279 @default.
• W768003421 hasConceptScore W768003421C182220744 @default.
• W768003421 hasConceptScore W768003421C185592680 @default.
• W768003421 hasConceptScore W768003421C2776414213 @default.
• W768003421 hasConceptScore W768003421C2776714187 @default.
• W768003421 hasConceptScore W768003421C2777807008 @default.
• W768003421 hasConceptScore W768003421C2900643 @default.
• W768003421 hasConceptScore W768003421C43617362 @default.
• W768003421 hasConceptScore W768003421C55493867 @default.
• W768003421 hasConceptScore W768003421C57409179 @default.
• W768003421 hasLocation W7680034211 @default.
• W768003421 hasOpenAccess W768003421 @default.
• W768003421 hasPrimaryLocation W7680034211 @default.
• W768003421 hasRelatedWork W1969153722 @default.
• W768003421 hasRelatedWork W1981936307 @default.
• W768003421 hasRelatedWork W1987342434 @default.
• W768003421 hasRelatedWork W1994746525 @default.
• W768003421 hasRelatedWork W2009653646 @default.
• W768003421 hasRelatedWork W2046166714 @default.
• W768003421 hasRelatedWork W2055333712 @default.
• W768003421 hasRelatedWork W2086512939 @default.
• W768003421 hasRelatedWork W2094511893 @default.
• W768003421 hasRelatedWork W2129548003 @default.
• W768003421 hasRelatedWork W2151704374 @default.
• W768003421 hasRelatedWork W2184962100 @default.
• W768003421 hasRelatedWork W2186449082 @default.
• W768003421 hasRelatedWork W2464192507 @default.
• W768003421 hasRelatedWork W2560527814 @default.
• W768003421 hasRelatedWork W2896817952 @default.
• W768003421 hasRelatedWork W2921883272 @default.
• W768003421 hasRelatedWork W1555441362 @default.
• W768003421 hasRelatedWork W2592314894 @default.
• W768003421 hasRelatedWork W3124692939 @default.
• W768003421 isParatext "false" @default.
• W768003421 isRetracted "false" @default.
• W768003421 magId "768003421" @default.
• W768003421 workType "dissertation" @default.