Matches in SemOpenAlex for { <https://semopenalex.org/work/W768609253> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W768609253 endingPage "56" @default.
- W768609253 startingPage "54" @default.
- W768609253 abstract "This study is conducted to clone the cDNA encoding human TNF-related apoptosis-inducing ligand(hTRAIL)extracellular region(amino acids 41-281,hTRAIL41-281)and to express it in E.coli.The hTRAIL41-281 cDNA is amplified by reverse transcription(RT)PCR from total RNA derived from human acute promyelocytic leukemia cell line HL-60.After sequenced,the cDNA is cloned into the vector pQE-80L and transformed into E.coli DH5α to express the recombinant hTRAIL41-281(rhTRAIL41-281)induced by IPTG.The recombinant protein is analyzed by SDS-PAGE.The cloned cDNA is consistent with the cDNA sequence encoding hTRAIL41-281 reported in GenBank^TM.After inducing.the hTRAIL41-281 protein is expressed,and the mass of the recombinant protein is about 30% of total bacteria protein,which demonstrates that the cDNA encoding hTRAIL41-281 is successfully cloned and expressed in E.coli." @default.
- W768609253 created "2016-06-24" @default.
- W768609253 creator A5011699100 @default.
- W768609253 creator A5027897543 @default.
- W768609253 creator A5087228533 @default.
- W768609253 date "2002-01-01" @default.
- W768609253 modified "2023-09-23" @default.
- W768609253 title "Expression of human TNF-related apoptosis-inducing ligand extracellular region in E.coli" @default.
- W768609253 hasPublicationYear "2002" @default.
- W768609253 type Work @default.
- W768609253 sameAs 768609253 @default.
- W768609253 citedByCount "0" @default.
- W768609253 crossrefType "journal-article" @default.
- W768609253 hasAuthorship W768609253A5011699100 @default.
- W768609253 hasAuthorship W768609253A5027897543 @default.
- W768609253 hasAuthorship W768609253A5087228533 @default.
- W768609253 hasConcept C104317684 @default.
- W768609253 hasConcept C153911025 @default.
- W768609253 hasConcept C170344550 @default.
- W768609253 hasConcept C187882448 @default.
- W768609253 hasConcept C28406088 @default.
- W768609253 hasConcept C40767141 @default.
- W768609253 hasConcept C55493867 @default.
- W768609253 hasConcept C79029880 @default.
- W768609253 hasConcept C86803240 @default.
- W768609253 hasConceptScore W768609253C104317684 @default.
- W768609253 hasConceptScore W768609253C153911025 @default.
- W768609253 hasConceptScore W768609253C170344550 @default.
- W768609253 hasConceptScore W768609253C187882448 @default.
- W768609253 hasConceptScore W768609253C28406088 @default.
- W768609253 hasConceptScore W768609253C40767141 @default.
- W768609253 hasConceptScore W768609253C55493867 @default.
- W768609253 hasConceptScore W768609253C79029880 @default.
- W768609253 hasConceptScore W768609253C86803240 @default.
- W768609253 hasIssue "2" @default.
- W768609253 hasLocation W7686092531 @default.
- W768609253 hasOpenAccess W768609253 @default.
- W768609253 hasPrimaryLocation W7686092531 @default.
- W768609253 hasRelatedWork W1935828301 @default.
- W768609253 hasRelatedWork W2009476325 @default.
- W768609253 hasRelatedWork W2018818407 @default.
- W768609253 hasRelatedWork W2026062362 @default.
- W768609253 hasRelatedWork W2038873619 @default.
- W768609253 hasRelatedWork W2039428188 @default.
- W768609253 hasRelatedWork W2042185014 @default.
- W768609253 hasRelatedWork W2046621497 @default.
- W768609253 hasRelatedWork W2053967656 @default.
- W768609253 hasRelatedWork W2054427827 @default.
- W768609253 hasRelatedWork W2058125110 @default.
- W768609253 hasRelatedWork W2114194173 @default.
- W768609253 hasRelatedWork W2191543670 @default.
- W768609253 hasRelatedWork W2274830845 @default.
- W768609253 hasRelatedWork W2412611650 @default.
- W768609253 hasRelatedWork W2515273182 @default.
- W768609253 hasRelatedWork W2204462539 @default.
- W768609253 hasRelatedWork W2415569719 @default.
- W768609253 hasRelatedWork W2704400804 @default.
- W768609253 hasRelatedWork W2868000363 @default.
- W768609253 hasVolume "1" @default.
- W768609253 isParatext "false" @default.
- W768609253 isRetracted "false" @default.
- W768609253 magId "768609253" @default.
- W768609253 workType "article" @default.